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Genetic and physical mapping of recF in Escherichia coli K-12

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Summary

Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou). Transductional analysis with a series of λtna specialized transducing phages carrying chromosomal DNA from the tnaA region place recF between dnaA and gyrB. This analysis also indicates that a gene lying in the same region and producing an easily detectable protein (estimated MW of 45 kD) is dnaN and not recF.

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Communicated by H.W. Boyer

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Ream, L.W., Margossian, L., Clark, A.J. et al. Genetic and physical mapping of recF in Escherichia coli K-12. Molec. Gen. Genet. 180, 115–121 (1980). https://doi.org/10.1007/BF00267359

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