Summary
Isolated protoplasts of Vicia faba and Petunia hybrida have been fused by the action of Ca++, high pH and, occasionally, PEG. The heterokaryotic stage frequently endured mitotic divisions. Only one cell was found with apparently fusing nuclei. Another heterokaryon showed asynchronous phases in the nuclear cycle. Three hybrid tissues have been identified 50 and 60 days after fusion. One of them could be propagated, is 9 months old and still rapidly proliferating. The fusion hybrids contained predominantly nuclei or chromosomes of one or the other species and a few chromosomes of the second parent. These observations were assigned to chromosome elimination which has been conclusively seen in cells containing nuclei of Petunia-type and one or two chromosomes of Vicia left in the cytoplasm. Breakage of chromosomes was the only type of chromosome mutations found in hybrid tissue.
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Communicated by G. Melchers
Dedicated with compliments to Professor Dr. Josef Straub on the occasion of his 67th birthday and the 17th anniversary of his directorship within the 50 years of history of the Erwin-Baur-Institut (Max-Planck-Institut für Züchtungsforschung)
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Binding, H., Nehls, R. Somatic cell hybridization of Vicia faba+Petunia hybrida . Molec. Gen. Genet. 164, 137–143 (1978). https://doi.org/10.1007/BF00267378
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DOI: https://doi.org/10.1007/BF00267378