Summary
The E. coli dnaK (groPC756) gene product is essential for bacteriophage λ DNA replication. Bacterial DNA segments carrying this gene have been cloned onto a bacteriophage λ vector. The product of the dnaK gene has been identified on SDS polyacrylamide gels after infection of UV-irradiated E. coli cells. The dnaK gene codes for a polypeptide with an apparent molecular weight of 93,000-Mr. Transducing phages carrying amber mutations in the dnaK gene fail to induce the synthesis of the 93,000-Mr polypeptide chain upon infection of sup + bacteria, but do so upon infection of supF bacteria. E. coli carrying the dnaK756 mutation are, in addition, temperature sensitive for growth at 43° C. It is shown that the dnaK756 mutation results in an overproduction of the dnaK gene product at that temperature.
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Georgopoulos, C.P., Lam, B., Lundquist-Heil, A. et al. Identification of the E. coli dnaK (groPC756) gene product. Molec. Gen. Genet. 172, 143–149 (1979). https://doi.org/10.1007/BF00268275
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DOI: https://doi.org/10.1007/BF00268275