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In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (JACQ) marechal

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Abstract

Callus cultures of two cultivars of Vigna aconitifolia (IPCMO-926, RDM-120) were raised and their growth and differentiation studied. In IPCMO-926 callus cultures, numerous shoot buds differentiated on MS medium with BA (0.4–22.2 μM) alone or in combination with IAA (5.7 μM). In RDM-120 best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM). Kinetin alone, however, induced rhizogenesis in callus cultures. In suspension cultures of IPCMO-926 embryoids differentiated on MS medium with K (0.5 μM) and 2,4-D (0.4 and 0.9 μM).

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Abbreviations

MS:

Murashige and Skoog (1962)

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-benzyladenine

IAA:

Indole-3-acetic acid

K:

Kinetin

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Authors and Affiliations

  1. Department of Botany, University of Rajasthan, 302004, Jaipur, India

    S. Bhargava & N. Chandra

Authors
  1. S. Bhargava
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  2. N. Chandra
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Bhargava, S., Chandra, N. In vitro differentiation in callus cultures of moth bean, Vigna aconitifolia (JACQ) marechal. Plant Cell Reports 2, 47–50 (1983). https://doi.org/10.1007/BF00269235

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  • DOI: https://doi.org/10.1007/BF00269235

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