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Transformation of Medicago by Agrobacterium mediated gene transfer

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Abstract

Shoot segments of Medicago varia genotype A2 were co-cultivated with Agrobacterium tumefaciens strain bo42 carrying pGA471, a plasmid coding for the kanamycin resistant determinant as transferable positive selection marker in plant cells (An et al., 1985). Resistant plants were regenerated at high frequency from green calli developed on inoculated stem cuttings under kanamycin selection. DNA-DNA hybridization analysis showed the presence of the structural gene of the kanamycin resistant determinant in total DNA isolated from several independent transformants. All data presented clearly demonstrate the transfer, stable maintenance and functional expression of the kanamycin resistance marker in Medicago varia cells which retain their morphogenic property.

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Abbreviations

Km:

kanamycin

KmR:

kanamycin resistant

Cb:

carbenicillin

2,4-D:

2,4 dichlorophenoxyacetic acid

BA:

6-benzyladenine

T-DNA:

transferred DNA into plants

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Author notes

  1. Csaba Koncz

    Present address: Max-Planck-Institut für Züchtungsforschung, D-5000, Köln 30, FRG

Authors and Affiliations

  1. Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, H-6701, Szeged, Hungary

    Maria Deak, Gyorgy B. Kiss, Csaba Koncz & Denes Dudits

Authors
  1. Maria Deak
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  2. Gyorgy B. Kiss
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  3. Csaba Koncz
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  4. Denes Dudits
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Communicated by A. Szalay

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Deak, M., Kiss, G.B., Koncz, C. et al. Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Reports 5, 97–100 (1986). https://doi.org/10.1007/BF00269243

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  • DOI: https://doi.org/10.1007/BF00269243

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