Abstract
Following ultraviolet mutagenesis, we have isolated two Na+ dependent variants from haploid suspension cultures of soybean cells. No selection was used in the isolation procedure. These cells cannot grow in 1 mM Na+ but can grow in 17 mM Na+. The strains also grow in 33 or 65 mM Na+, concentrations in which the parent cell line dies. [22Na] was used to measure the accumulation of Na+ by parent and variant cell lines. Two- to three-fold less Na+ is retained by the variant cell lines.
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This work was supported by a grant from National Institute of Environmental Health Sciences (01 498) to K.G.L.
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Jia-Ping, Z., Roth, E.J., Terzaghi, W. et al. Isolation of sodium dependent variants from haploid soybean cell culture. Plant Cell Reports 1, 48–51 (1981). https://doi.org/10.1007/BF00269269
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DOI: https://doi.org/10.1007/BF00269269