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Sterol glucosylation by the plasma membrane from cotyledons of Phaseolus aureus

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Abstract

Membrane fractions were isolated from dark grown cotyledons of Phaseolus auneus by differential and sucrose density gradient centrifugation. Endoplasmic reticulum-, Golgi apparatus- and plasma membrane-rich fractions were identified by their respective enzymic activities and tested for their ability to transfer glucose from UDP-glucose to endogenous sterols to form steryl glucosides. The glucosyltransferase activity was shown to be located mainly at the plasma membrane.

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Abbreviations

SG:

steryl glucoside

ASG:

acylated steryl glucoside

UDP-glc:

Uridine diphosphoglucose

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Authors and Affiliations

  1. Laboratoire de Biochimie Végétale, Institut de Botanique de l'Université Louis Pasteur, 28, rue Goethe

    Marie-Andrée Hartmann-Bouillon, Bernard Walter & Eliane Lazar

  2. Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15, rue Descartes, F-6700, Strasbourg, France

    Marie-Andrée Hartmann-Bouillon, Bernard Walter & Eliane Lazar

Authors
  1. Marie-Andrée Hartmann-Bouillon
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  2. Bernard Walter
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  3. Eliane Lazar
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Hartmann-Bouillon, MA., Walter, B. & Lazar, E. Sterol glucosylation by the plasma membrane from cotyledons of Phaseolus aureus . Plant Cell Reports 1, 56–58 (1981). https://doi.org/10.1007/BF00269271

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  • DOI: https://doi.org/10.1007/BF00269271

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