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Somatic embryos from callus of Sequoia sempervirens

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Abstract

Compact calli with a potential for somatic embryogenesis were obtained from complete or split mature zygotic embryos or from cotyledons and hypocotyls of in vitro grown seedlings of Sequoia sempervirens. Somatic embryos which showed a typical bipolar structure, were formed together with adventitious buds. When placed on filter paper supports they developed into complete plantlets. Of the various combinations tested, culture medium adapted from Murashige and Skoog mineral solution complemented with 6-benzylaminopurine (2 μM), kinetin (2 μM) and 2,4-dichlorophenoxyacetic acid (2.5 μM) was established as the optimal for somatic embryo production.

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Abbreviations

BM:

basic medium

2,4-D:

dichlorophenoxyacetic acid

BAP:

6-benzylaminopurine

Kin:

Kinetin

References

  • Boulay M (1978) In: Annales AFOCEL 1977, pp. 37–67.

  • Buchholz JT (1939) Am. J. Bot. 26: 248–257.

    Google Scholar 

  • Dameri RM, Caffaro L, Gastaldo P, Profumo P (1986) J. Plant Physiol. 136: 93–96.

    Google Scholar 

  • Fouret Y, Arnaud Y, Larrieu C (1985) In: Annales AFOCEL 1984, pp. 111–138.

  • Gonzalez ML, Vieitez AM, Vieitez E (1985) Scientia Horticulturae, 27: 97–103.

    Google Scholar 

  • Gupta PK Durzan DJ (1986 a) Bio/Technology, 4: 643–645.

    Google Scholar 

  • Gupta PK Durzan DJ (1986 b) In Vitro Cellular and Developmental Biology, 22: 685–688.

    Google Scholar 

  • Haccius B (1978) Phytomorphology, 28: 74–81.

    Google Scholar 

  • Hakman I, Fowke LC (1987) Can. J. Bot. 65: 656–659.

    Google Scholar 

  • Hakman I, Von Arnold S (1985) J. Plant Physiol. 121: 149. 158

    Google Scholar 

  • Hakman I, Fowke LC, Von Arnold S, Eriksson T (1985) Plant Science 38:53–59

    Google Scholar 

  • Karpentschenko (1927) Hereditas, IX, 349.

    Google Scholar 

  • Krogstrup P (1986) Can. J. For. Res. 16: 664–668.

    Google Scholar 

  • Lelu MA, Boulay M, Arnaud Y (1987) C. R. Acad. Sci. Paris, t 305, série III: 105–109.

    Google Scholar 

  • Reinert J (1973) In: HE Street (Editor), Plant tissue and cell culture, Blackwell, Oxford pp. 338–355.

    Google Scholar 

  • Von Arnold S, Hakman I (1986) J. Plant Physiol. 122: 261–265.

    Google Scholar 

  • Walker (1986) In: Annales AFOCEL 1985, pp. 25–47.

Download references

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Authors and Affiliations

  1. Laboratoire de Biologie des Ligneux, JE C.N.R.S. 034613, Université de Nancy I, B.P. 239, F-54506, Vandoeuvre Cedex, France

    F. Bourgkard & J. M. Favre

Authors
  1. F. Bourgkard
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  2. J. M. Favre
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Communicated by F. Constabel

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Bourgkard, F., Favre, J.M. Somatic embryos from callus of Sequoia sempervirens . Plant Cell Reports 7, 445–448 (1988). https://doi.org/10.1007/BF00269534

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  • DOI: https://doi.org/10.1007/BF00269534

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