Skip to main content
SpringerLink
Log in

Somatic embryogenesis from cultured leaf segments of Zea mays

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Basal leaf segments of 3 to 4 week old maize (Zea mays L.) seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos. Histological evidence showed that some of the embryos arose directly from the explant. When leaf segments with embryos were transferred to MS medium with 1.0 μM NAA, 1.0 μM IAA, 2.0 μM 2iP, and 60 g/l sucrose, the embryos germinated and the resulting seedlings could be established in culture tubes. These responses were obtained from three inbred lines, CHI31, S615, and S7.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

SH:

Schenk and Hildebrandt (1972) medium

MS:

Murashige and Skoog (1962) medium

dicamba:

3,6-dichloro-o-anisic acid

IAA:

indole-3-acetic acid

NAA:

α-naphthaleneacetic acid

2iP:

2-isopentyladenine

References

  • Ahlquist P, French R, Janda M, Loesch-Fries SL (1984) Proc. Natl. Acad. Sci. (USA) 81:7066–7070.

    Google Scholar 

  • Armstrong CL, Green CE (1985) Planta 164:207–214.

    Google Scholar 

  • Conger, BV, Hanning GE, Gray DJ, McDaniel JK (1983) Science 221:850–851.

    Google Scholar 

  • Evans DA, Sharp WR (1986) In: Evans DA, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture, vol 4, Techniques and Applications, Macmillan Publishing Co., New York, pp 97–132.

    Google Scholar 

  • Göbel E, Brown PTH, Lörz H (1986) In: Nuclear techniques and in vitro culture for plant improvement. Intern. Atomic Energy Agency, Vienna, pp 21–28.

    Google Scholar 

  • Grimsley N, Hohn T, Davies JW, Hohn B (1987) Nature 325:177–179.

    Google Scholar 

  • Hanning GE, Conger BV (1982) Theor. Appl. Genet. 63:155–159.

    Google Scholar 

  • Hodges TK, Kamo KK, Imbrie CW, Becwar MR (1986) Bio/Technology 4:219–223.

    Google Scholar 

  • Horsch RB, Fry JE, Hoffman NL, Eicholtz D, Rogers SG, Fraley RT (1985) Science 227:1229–1231.

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Theor. Appl. Genet. 60:197–214.

    Google Scholar 

  • Lörz H, Baker B, Junker B, Matzeit V, Göbel E (1986) In: Nuclear techniques and in vitro culture for plant improvement. Intern. Atomic Energy Agency, Vienna, pp 505–509.

    Google Scholar 

  • Lu C, Vasil IK, Ozias-Akins P (1982) Theor. Appl. Genet. 62:109–112.

    Google Scholar 

  • McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Plant Cell Reports 5:81–84.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497.

    Google Scholar 

  • Novak FJ, Dolezelova M, Nesticky M, Piovarci A (1983) Maydica 28:381–390.

    Google Scholar 

  • Novak FJ, Hermelin T, Daskalov S (1986) In: Horn W, Jensen CJ, Odenbach W, Schieder O (eds) Genetic manipulation in plant breeding. Walter de Gruyter & Co., Berlin, pp 563–576.

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972) Can. J. Bot. 50:199–204.

    Google Scholar 

  • Songstad DD, Conger BV (1986) Amer. J. Bot. 73:989–992.

    Google Scholar 

  • Vasil V, Vasil IK (1986) J. Plant Physiol. 124:399–408.

    Google Scholar 

  • Wernicke W, Brettell (1980) Nature 287:138–139.

    Google Scholar 

Download references

Author information

Author notes

  1. B. V. Conger

    Present address: Department of Plant and Soil Science, University of Tennessee, P.O. Box 1071, 37901, Knoxville, TN, USA

  2. F. J. Novak

    Present address: Institute of Experimental Botany, Olomonc, Czechoslovakia

Authors and Affiliations

  1. Plant Breeding and Genetics Unit, FAO/IAEA Agricultural Programme, International Atomic Energy Agency, Seibersdorf Laboratory, P.O. Box 100, A-1400, Vienna, Austria

    B. V. Conger, F. J. Novak & R. Afza

  2. Department of Plant Physiology, Comenius University, Bratislava, Czechoslovakia

    K. Erdelsky

Authors
  1. B. V. Conger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. J. Novak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Afza
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Erdelsky
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Lörz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Conger, B.V., Novak, F.J., Afza, R. et al. Somatic embryogenesis from cultured leaf segments of Zea mays . Plant Cell Reports 6, 345–347 (1987). https://doi.org/10.1007/BF00269556

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00269556

Keywords

Search

Navigation