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A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain)

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Abstract

A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions is presented in this paper. Shoot-tip cultures of Musa cultivars (both banana and plantain) are induced by culturing small excised shoot apices on modified MS semisolid medium supplemented with various concentrations and combinations of auxins and cytokinins. The effects of cytokinin concentration in the medium as well as the genotypic configuration of the cultivars on the rate of shoot-bud proliferation have been tested. The established shoot-tip cultures grown on modified MS semisolid medium supplemented with IAA (0.18 mg/l) and BA (2.30 mg/l) have been successfully stored at 15°C with 1000 lux light intensity up to 13–17 months depending on the cultivar. The cultivars tested in the present investigation seem to vary in their ability to withstand minimal growth temperature.

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Abbreviations

BA:

Benzyladenine

IAA:

Indoleacetic acid

IBA:

Indolebutyric acid

MS:

Murashige and Skoog

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Author notes

  1. Nirmalya Banerjee

    Present address: Centre of Advance Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India

Authors and Affiliations

  1. Laboratorium voor Teeltfysiologie en Tropische Fytotechnie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B-3030, Leuven, Belgium

    Nirmalya Banerjee & Edmond de Langhe

Authors
  1. Nirmalya Banerjee
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  2. Edmond de Langhe
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Communicated by D. von Wettstein

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Banerjee, N., de Langhe, E. A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (Banana and plantain). Plant Cell Reports 4, 351–354 (1985). https://doi.org/10.1007/BF00269897

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  • DOI: https://doi.org/10.1007/BF00269897

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