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Transformation of cotton (Gossypium hirsutum L.) via particle bombardment

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Abstract

Embryogenic suspension cultures of cotton (Gossypium hirsutum L.) were subjected to particle bombardment, where high density particles carrying plasmid DNA were accelerated towards the embryogenic plant cells. The plasmid DNA coating the particles encoded hygromycin resistance. One to two weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing 100 μg/ml hygromycin. Clumps of tissue which grew in the presence of hygromycin were subcultured at low density into fresh hygromycin-containing proliferation medium. Following sequential transfer of embryogenic tissue to development and then germination media, plants were recovered from transgenic embryogenic tissue. Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

GUS:

β-glucuronidase

Aph IV:

aminoglycoside phosphotransferase type IV

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Authors and Affiliations

  1. Department of Agronomy and Ohio State Biotechnology Center, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA

    John J. Finer & Michael D. McMullen

  2. Corn and Soybean Research Unit, Agricultural Research Service, USDA, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA

    Michael D. McMullen

Authors
  1. John J. Finer
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  2. Michael D. McMullen
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Additional information

Communicated by G. C. Phillips

Salaries and research support were provided by State and Federal funds appropriated to OSU/OARDC and USDA-ARS. Mention of trademark or proprietary products does not constitute a guarantee or warranty of the product by OSU/OARDC or USDA, and also does not imply approval to the exclusion of other products that may also be suitable. Journal Article No. 354-89

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Finer, J.J., McMullen, M.D. Transformation of cotton (Gossypium hirsutum L.) via particle bombardment. Plant Cell Reports 8, 586–589 (1990). https://doi.org/10.1007/BF00270059

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  • DOI: https://doi.org/10.1007/BF00270059

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