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Genes for the fermentation of maltose and α-methylglucoside in Saccharomyces carlsbergensis

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Summary

  1. 1.

    Saccharomyces carlsbergensis N.C.Y.C.74 was shown to be diploid and heterothallic. On acetate medium four spored asci were produced with a spore viability of no more than 2%. However diploid hybrids from the viable spores yielded asci with a spore viability of 75%, thus making tetrad analysis and consequently a genetical study of sugar fermentation in this strain feasible.

  2. 2.

    Auxotrophic mutants in a haploid derivative of this strain were obtained, some of which could be classified as ade 1, ade 2, ade 4, ade 5, ade 6, ade 7, ura 2, ura 3, lys 1, trp 2, trp 5, his 4 and his 5.

  3. 3.

    150 Random spores all fermented glucose, sucrose, mannose, galactose, melibiose, raffinose, and maltose, but only 28% fermented α-methylglucoside. The same fermentation pattern was found in petites of these 150 spores, except that 40% grew poorly on galactose; this gal phenotype could not be ascribed to a single gene.

  4. 4.

    In crosses with mal strains the maltose fermenting ability of the spores from S. carlsbergensis appeared to be dependent upon 1 single gene, which could be identified as MAL 6, one of the 7 known polymeric genes for maltose fermentation in Saccharomyces.

  5. 5.

    Our strain was found to be heterozygous for 2 unlinked MGL genes, in this paper indicated as MGL a, and MGL b, both indispensable for the fermentation of α-methylglucoside.

  6. 6.

    After mutagenesis of a haploid strain with genotype MGL aMGLb, one mgl mutant was found, which complemented a strain with genotype mgl amglb. This third indispensable MGL gene, preliminarily called MGL c, is homozygously present in our strain and is not linked to either MGL a or MGL b.

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References

  • Bloemers, H. P. J.: Induced α-glucosidase synthesis in Saccharomyces carlsbergensis. Thesis, Utrecht, 1967.

  • Burton, K.: A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochem. J. 62, 315–323 (1956).

    Google Scholar 

  • Douglas, H., Pelroy, G.: A gene controlling the inducibility of the galactose pathway enzymes in Saccharomyces. Biochim. biophys. Acta (Amst.) 68, 155–156 (1963).

    Google Scholar 

  • Emeis, C., Gutz, H.: Eine einfache Technik zur Massenisolation von Hefesporen. Z. Naturforsch. 13b, 647–650 (1958).

    Google Scholar 

  • Fowell, R.: Sodium acetate agar as a sporulation medium for yeast. Nature (Lond.) 170, 578 (1952).

    Google Scholar 

  • Gilliland, R.: Identification of the genes for maltose fermentation in Saccharomyces diastaticus. Nature (Lond.) 173, 409 (1954).

    Google Scholar 

  • Gorman, J. W.: The biosynthetic control of α-glucosidase and isomaltase in genetically defined strains of Saccharomyces cerevisiae. Thesis, University of Wisconsin, 1963.

  • Hawthorne, D. C.: Chromosome mapping in Saccharomyces. Thesis, University of Washington, Seatte, U.S.A., 1955.

    Google Scholar 

  • Hawthorne, D. C.: The genetics of α-methylglucoside fermentation in Saccharomyces. Heredity 12, 273–284 (1958).

    Google Scholar 

  • Khan, N.: Biochemical and genetic studies of maltase and α-methylglucosidase formation in yeast. Thesis, The City University of New York, 1967.

  • Koningsberger, V. V.: The regulation of inducible α-glucosidase synthesis in yeast. In: L. Bosch and V. V. Koningsberger, Regulation of nucleic acid and protein biosynthesis. Amsterdam: Elsevier Publishing Cy 1967.

    Google Scholar 

  • Kroon, R. de, Koningsberger, V. V.: An inducible transport system for α-glucosides in protoplasts of Saccharomyces carlsbergensis. Biochim. biophys. Acta (Amst.) 204, 590–609 (1970).

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).

    Google Scholar 

  • Mortimer, R. K., Hawthorne, D. C.: Genetic mapping in Saccharomyces. Genetics 53, 165–173 (1966).

    Google Scholar 

  • Mortimer, R. K., Hawthorne, D. C.: Yeast genetics. In: H. Rose and J. S. Harrison, The yeasts. p. 385–460, London and New York: Academic Press 1969.

    Google Scholar 

  • Nagai, S.: Induction of the respiration deficient mutation in yeast by various synthetic dyes. Science 130, 1188–1189 (1959).

    Google Scholar 

  • Oeser, H.: Genetische Untersuchungen über das Paarungstypverhalten bei Saccharomyces und die Maltose-gene einiger untergäriger Bierhefen. Arch. Mikrobiol. 44, 47–74 (1962).

    Google Scholar 

  • Oeser, H., Windisch, S.: Die Bestimmung eines weiteren Maltose-Gens bei Hefen der Gattung Saccharomyces. Naturwissenschaften 51, 122 (1964).

    Google Scholar 

  • Ogur, M., Minckler, S., Lindegren, G., Lindegren, C. C.: The nucleic acids in a polyploid series of Saccharomyces. Arch. Biochem. 40, 175–184 (1952).

    Google Scholar 

  • Okada, H., Halvorson, H. O.: Uptake of α-thioethyl-D-glucopyranoside by Saccharomyces cerevisiae: I. The genetic control of facilitated diffusion and active transport. Biochim. biophys. Acta (Amst.) 82, 538–546 (1964a).

    Google Scholar 

  • Okada, H., Halvorson, H. O.: II. General characteristics of an active transport system. Biochim. biophys. Acta (Amst.) 82, 547–555 (1964b).

    Google Scholar 

  • Oshima, Y.: The intercistronic complementation of the polymeric genes for maltose fermentation in Saccharomyces. J. Fermen. Technol. (Japan) 46, 550–565 (1967).

    Google Scholar 

  • Perkins, D. D.: Biochemical mutants in the smut fungus Ustilago maydis. Genetics (Princeton) 34, 607–626 (1949).

    Google Scholar 

  • Poll, K. W. van de: Regulation of allantoinase synthesis in Saccharomyces carlsbergensis. Thesis, Utrecht 1969.

  • Roman, H.: A system selective for mutations affecting the synthesis of adenine in yeast. C. R. Lab. Carlsberg 26, 299–314 (1956).

    Google Scholar 

  • Spiegelman, S., Sussman, R., Pinska, E.: On the cytoplasmic nature of “long-term adaptation” in yeast. Proc. nat. Acad. Sci. (Wash.) 36, 591–606 (1950).

    Google Scholar 

  • Takahashi, T., Ikeda, Y.: Genetic analysis of α-methylglucoside fermentation in Saccharomyces. Z. Vererbungsl. 90, 66–73 (1959).

    Google Scholar 

  • Terui, G., Okada, H., Oshima, Y.: Studies on the correlation of α-glucosidase formation with genotypic composition in Saccharomyces. Techn. Rep. Osaka Univ. 9, 237–259 (1959).

    Google Scholar 

  • Winge, Ø, Roberts, C.: The polymeric genes for maltose fermentation in yeasts and their mutability. C.R. Lab. Carlsberg 25, 36–83 (1950a).

    Google Scholar 

  • Winge, Ø, Roberts, C.: Identification of the gene for maltose fermentation in Saccharomyces italicus. Nature (Lond.) 166, 1114 (1950b).

    Google Scholar 

  • Winge, Ø., Roberts, C.: The relation between the polymeric genes for maltose, raffinose and sucrose fermentation in yeasts. C.R. Lab. Carlsberg, 25, 141–171 (1952).

    Google Scholar 

  • Winge, Ø., Roberts, C.: Identification of the maltase genes in some American haploid and European diploid yeasts. C.R. Lab. Carlsberg 25, 331–340 (1955).

    Google Scholar 

  • Wijk, R. van: α-Glucosidase synthesis, respiratory enzymes and catabolite repression in yeast. Thesis, Utrecht 1968.

  • Wijk, R. van, Konijn, T. M.: Cyclic 3′, 5′-AMP in Saccharomyces carlsbergensis under various conditions of catabolite repression. FEBS Letters 13, 184–186 (1971).

    Google Scholar 

  • Zimmermann, F. K., Schwaier, R.: Induction of mitotic gene conversion with nitrous acid, 1-methyl-3-nitro-1-nitroso-guanidine and other alkylating agents in Saccharomyces cerevisiae. Molec. gen. Genet. 100, 63–76 (1967).

    Google Scholar 

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  1. Van't Hoff Laboratory, sterrenbos 19, Utrecht, The Netherlands

    A. M. A. ten Berge

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  1. A. M. A. ten Berge
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Communicated by F. Kaudewitz

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ten Berge, A.M.A. Genes for the fermentation of maltose and α-methylglucoside in Saccharomyces carlsbergensis . Molec. Gen. Genet. 115, 80–88 (1972). https://doi.org/10.1007/BF00272220

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  • DOI: https://doi.org/10.1007/BF00272220

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