Summary
Total endosperm protein subunits, extracted from the common wheat cultivar Chinese Spring and from some of its aneuploid lines, were fractionated according to their molecular weight (MW) in an improved high resolution one-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The resolution obtained by this method and, in particular, that of the high molecular weight (HMW) glutenin and gliadin subunits approached that of a previous report in which two-dimensional fractionation system based on charge and MW was used. In the cultivar Chinese Spring, 21 discrete protein bands were resolved and the chromosomes controlling many of them were either reconfirmed, or, in some cases, established. The advantages of this high resolution SDS PAGE technique are discussed.
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Communicated by J. MacKey
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Galili, G., Feldman, M. Genetic control of endosperm proteins in wheat. Theoret. Appl. Genetics 64, 97–101 (1983). https://doi.org/10.1007/BF00272711
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DOI: https://doi.org/10.1007/BF00272711