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Assay of plant proteins with bicinchoninic acid for high resolution two-dimensional polyacrylamide gel electrophoresis

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Abstract

A method is described for estimating proteins in the same plant tissue sample that is solubilized for separation by two-dimensional polyacrylamide gel electrophoresis. The method uses a modified bicinchoninic acid (BCA) protein assay procedure and a modified standard urea solubilization buffer to estimate microgram values of unknown protein concentration, in the presence of 9 M urea and 4% Nonidet P-40, from a linear standard curve. A method for a quantitative determination of protein concentration by BCA in a sample containing 9 M urea and 4% Nonidet P-40 is also described. This method is effective for the determination of proteins in minute non-green and green plant tissue, and is especially designed for vegetative and floral shoot apices, and the primordia of inflorescences.

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Authors and Affiliations

  1. Department of Biology, University of Northern Iowa, IA 50614, Cedar Falls, Iowa, USA

    Alan R. Orr, Brett A. Wagner, Catherine T. Howard & Orlando A. Schwartz

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  1. Alan R. Orr
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  2. Brett A. Wagner
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  3. Catherine T. Howard
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  4. Orlando A. Schwartz
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Communicated by I. K. Vasil

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Orr, A.R., Wagner, B.A., Howard, C.T. et al. Assay of plant proteins with bicinchoninic acid for high resolution two-dimensional polyacrylamide gel electrophoresis. Plant Cell Reports 7, 598–601 (1988). https://doi.org/10.1007/BF00272767

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  • DOI: https://doi.org/10.1007/BF00272767

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