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Protection and host cell repair of irradiated lambda phage

III. Ultraviolet Irradiation of 5-bromouracil-substituted phage

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Summary

5-bromouracil-containing λ phage, irradiated with UV light in the presence and absence of cysteamine, were assayed on normal and DNA-repair deficient bacteria.

It has been found that these phage suffer (1) lesions, of unknown nature, which are neither reparable by the bacterial DNA repair systems nor prevented by cysteamine, a free radical scavenger and proton donor; (2) lesions which are converted to reparable lesions by cysteamine, and which may be sugar damages (perhaps accompanied by pyridimine dimers) which are converted to simple dimers; and (3) irreparable lesions, which do not occur in the presence of cysteamine, and which are probably sugar damages. These latter are the most important, and in large part explain the increased sensitivity to irradiation of 5-bromouracil-substituted phage.

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References

  • Aoki, S., R. P. Boyce, and P. Howard-Flanders: Sensitization of Escherichia coli to radiation by bromouracil: excessive post-irradiation breakdown of deoxyribonucleic acid without concomitant synthesis. Nature (Lond.) 209, 686 (1966).

    Google Scholar 

  • Boyce, R. P.: Production of additional sites of deoxyribonucleic acid breakdown in bromouracil containing Escherichia coli exposed to ultraviolet light. Nature (Lond.) 209, 688 (1966).

    Google Scholar 

  • Echols, H., and R. Gingery: Mutants of bacteriophage λ defective in vegetative genetic recombination. J. molec. Biol. 34, 239 (1968).

    Google Scholar 

  • Hotz, G.: Suppression by cysteamine of radiosensitization in 5-bromodeoxyuridine substituted phage T 1. Biochem. biophys. Res. Commun. 11, 393 (1963).

    Google Scholar 

  • —, and H. Reuschl: Damage to deoxyribose molecule and to U-gene reactivation in UV-irradiated 5-bromouracil DNA of phage T 4 Bor as influenced by cysteamine. Molec. Gen. Genetics 99, 5 (1967).

    Google Scholar 

  • Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175 (1968).

    Google Scholar 

  • —, R. P. Boyce, and L. Theriot: Mechanism of sensitization to ultra-violet light of T 1 bacteriophage by incorporation of 5-bromodeoxyuridine or by pre-irradiation of the host cell. Nature (Lond.) 195, 51 (1962).

    Google Scholar 

  • Hutchinson, F.: Private communication, 1967.

  • Koehnlein, W., and F. Hutchinson: ESR studies of normal and bromouracil substituted transforming DNA of Bacillus subtilis after irradiation with ultraviolet light. 3rd Int. Congr. Radiat. Res., Cortina d'Ampezzo 1966.

  • Radman, M., A. Roller, and M. Errera: Protection and host cell repair of irradiated lambda phage I. Irradiation of normal phage with ultraviolet light. Molec. Gen. Genetics 104 (1969).

  • Radman, M., A. Roller, and M. Errera: Protection and host cell repair of irradiated lambda phage II. Irradiation of 5-bromouracil-substituted phage with near visible light. Molec. Gen. Genetics 104 (1969).

  • Sauerbier, W.: The influence of 5-bromodeoxyuridine substitution on UV-sensitivity, host-cell reactivation and photoreactivation in T 1 and P 22 H 5. Virology 15, 465 (1961).

    Google Scholar 

  • Signer, E. R., and J. Weil: Recombination in bacteriophage λ. I. Mutants deficient in general recombination. J. Molec. Biol. 34, 261 (1968).

    Google Scholar 

  • Stahl, F. W., J. M. Craseman, L. Okun, E. Fox, and C. Laird: Radiation sensitivity of bacteriophage containing 5-bromodeoxyuridine. Virology 13, 98 (1961).

    Google Scholar 

  • Wacker, A.: Strahlenchemische Veränderungen von Pyrimidinen in vivo und in vitro. J. Chim. Phys. 58, 1041 (1961)

    Google Scholar 

  • Weiss, B., and C. C. Richardson: Enzymatic breakage and joining of deoxyribonucleic acid. I. Repair of single-stranded breaks in DNA by an enzyme system from Escherichia coli infected with T 4 bacteriophage. Proc. nat. Acad. Sci. (Wash.) 51, 1021 (1967).

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biophysique et Radiobiologie, Faculté des Sciences, Université libre de Bruxelles, Bruxelles, Belgium

    M. Radman (Research fellow of European Molecular Biology Organization), A. Roller & M. Errera

Authors
  1. M. Radman
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  2. A. Roller
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  3. M. Errera
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Additional information

Communicated by P. Slonimski

Biochemistry Department, College of Physicians and Surgeons Columbia University, New York City, New York

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Radman, M., Roller, A. & Errera, M. Protection and host cell repair of irradiated lambda phage. Molec. Gen. Genet. 104, 152–156 (1969). https://doi.org/10.1007/BF00272796

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  • DOI: https://doi.org/10.1007/BF00272796

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