Summary
Total ribosomal protein from rat liver ribosomes can be separated into about 20 chief electrophoretic fractions by preparative polyacrylamide gel electrophoresis. Ten electrophoretically homogeneous fractions have been isolated from the total mixture of ribosomal protein, respectively from proteins, prefractionated by CM-cellulose chromatography. Amino acid composition and molecular weights of some fractions have been determined. The amino acid composition of these fractions and of the total protein mixture are basically similar but there are also significant differences with regard to some amino acids. The molecular weights of the proteins studied are in the range between 7,000 and 29,000.
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Communicated by H. G. Wittmann
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Welfle, H., Bielka, H. & Böttger, M. Studies on proteins of animal ribosomes. Molec. Gen. Genet. 104, 165–171 (1969). https://doi.org/10.1007/BF00272798
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DOI: https://doi.org/10.1007/BF00272798