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Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombe

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Summary

Nine radiation-sensitive mutants of S. pombe showing a variety of phenotypic characteristics were analysed for their ability to excise pyrimidine dimers after ultraviolet irradiation. From earlier studies using indirect parameters, it was expected that some would be excision-deficient. Data reported here show that all the mutants tested, like wild type cells, were able to remove a high percentage of pyrimidine dimers during post-irradiation incubation in several different holding media, but not in saline or phosphate buffer. These mutants included strains showing increased, as well as others which showed decreased, levels of UV-induced mutation frequency relative to that of the wild type at the same total dose.

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References

  • Birnboim, H. C.: New method for extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe. J. Bact. 107, 659–663 (1971)

    Google Scholar 

  • Birnboim, H. C., Nasim, A.: Detection of pyrimidine dimers in hydrolysates of yeast DNA by high voltage paper electrophoresis. Molec. gen. Genet. 130, 291–296 (1974)

    Google Scholar 

  • Clarke, C. H.: Differential effects of caffeine in mutagen-treated Schizosaccharomyces pombe. Mutation Res. 5, 33–40 (1968)

    Google Scholar 

  • Fabre, F.: A UV-super sensitive mutant in the yeast Schizosaccharomyces pombe. Evidence for two repair pathways. Molec. gen. Genet. 110, 134–143 (1971)

    Google Scholar 

  • Fabre, F.: Relation between repair mechanisms and induced mitotic recombination after UV irradiation in the yeast Schizosaccharomyces pombe: effects of caffeine. Molec. gen. Genet. 117, 153–166 (1972)

    Google Scholar 

  • Fabre, F., Moustacchi, E.: Removal of pyrimidine dimers in cells of Schizosaccharomyces pombe mutated in different repair pathways. Biochim. biophys. Acta (Amst.) 312, 617–625 (1973)

    Google Scholar 

  • Hill, R. F.: Ultraviolet-induced lethality and reversion to prototrophy in Escherichia coli strains with normal and reduced dark repair ability. Photochem. Photobiol. 4, 563–568 (1965)

    Google Scholar 

  • Lawrence, C. W., Stewart, J. W., Sherman, F., Christensen, R.: Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome C ochre mutant in radiation sensitive strains of yeast. J. molec. Biol. 85, 137–162 (1974)

    Google Scholar 

  • Loprieno, N.: UV radiation-sensitivity and mutator activity in yeast Schizosaccharomyces pombe in Proc. International Congress of Radiation Research, 4th, Evian-les-Baines, France, 1970. Advances in radiation research: physics and chemistry. Editors: J. F. Duplan and A. Chapiro. New York: Gordon and Breach 1973

    Google Scholar 

  • Loprieno, N., Barale, R., Baroncelli, S.: Genetic effects of caffeine. Mutation Res. 26, 83–87 (1974)

    Google Scholar 

  • Loprieno, N., Schüpbach, M.: On the effect of caffeine on mutation and recombination in Schizosaccharomyces pombe. Molec. gen. Genet. 110, 348–354 (1971)

    Google Scholar 

  • Nasim, A.: Repair-mechanisms and radiation-induced mutations in fission yeast. Genetics 59, 329–333 (1968)

    Google Scholar 

  • Nasim, A.: Spontaneous and UV-induced mutations in radiation sensitive strains of fission yeast. Genetics 74, S 191–192 (1973)

    Google Scholar 

  • Nasim, A.: UV-induced replicating instability in Schizosaccharomyces pombe. Mutation Res. 22, 25–31 (1974)

    Google Scholar 

  • Nasim, A., Clark, C. H.: Nitrous acid-induced mosaicism in Schizosaccharomyces pombe. Mutation Res. 2, 395–402 (1965)

    Google Scholar 

  • Nasim, A., Smith, B. P.: Dark repair inhibitors and pathways for repair of radiation damage in Schizosaccharomyces pombe. Molec. gen. Genet. 133, 13–22 (1974)

    Google Scholar 

  • Nasim, A., Smith, B. P.: Genetic control of radiation sensitivity in Schizosaccharomyces pombe. Submitted for publication (1974b)

  • Resnick, M. A.: Induction of mutations in Saccharomyces cerevisiae by ultraviolet light. Mutation Res. 7, 315–332 (1969)

    Google Scholar 

  • Resnick, M. A., Setlow, J. K.: Repair of pyrimidine dimer damage induced in yeast by ultraviolet light. J. Bact. 109, 979–986 (1972)

    Google Scholar 

  • Schüpbach, M.: The isolation and genetic classification of UV-sensitive mutants of Schizosaccharomyces pombe. Mutation Res. 11, 361–371 (1971)

    Google Scholar 

  • Unrau, P., Wheatcroft, R., Cox, B. S.: The excision of pyrimidine dimers from DNA of ultraviolet irradiated yeast. Molec. gen. Genet. 113, 359–362 (1971)

    Google Scholar 

  • Waters, R., Moustacchi, E.: The disappearance of ultraviolet-induced pyrimidine dimers from the nuclear DNA of exponential and stationary phase cells of Saccharomyces cerevisiae following various post-irradiation treatments. Biochim. biophys. Acta (Amst.) 353, 407–419 (1974)

    Google Scholar 

  • Witkin, E. M.: Radiation-induced mutations and their repair. Science 152, 1345–1353 (1966a)

    Google Scholar 

  • Witkin, E. M.: Mutation-proof and mutation prone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light. In: Recovery and repair mechanisms in radiobiology. Brookhaven Symposia in Biology 20, 525–552 (1967)

  • Witkin, E. M., George, D. L.: Ultraviolet mutagenesis in pol A and uvr A pol A derivatives of Escherichia coli B/r: evidence for an inducible error-prone repair system. Genetics, Suppl. 73, 91–108 (1973)

    Google Scholar 

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Authors and Affiliations

  1. Biology and Health Physics Division, Atomic Energy of Canada Limited, Chalk River, Ontario, Canada

    H. C. Birnboim

  2. Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada

    A. Nasim

Authors
  1. H. C. Birnboim
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  2. A. Nasim
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Communicated by Ch. Auerbach

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Birnboim, H.C., Nasim, A. Excision of pyrimidine dimers by several UV-sensitive mutants of S. pombe . Molec. Gen. Genet. 136, 1–8 (1975). https://doi.org/10.1007/BF00275444

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  • DOI: https://doi.org/10.1007/BF00275444

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