Abstract
A simple technique of autologous platelet labelling is described, which allows labelling within 40 min, and has the advantage of low costs, as no laminar air flow is required. Blood (16 ml) was withdrawn into 4 ml ACD, 500 ng prostacyclin was added. After 10 min sedimentation the vials were centrifuged for 5 min at 150 g. The plateletrich plasma in the supernatant was centrifuged at 500 g for 10 min to obtain a platelet pellet. The platelet-poor plasma was preserved in a sterile syringe and the platelet pellet was resuspended in 1 ml tyrode buffer. The cell suspension was labelled at 37° C for 5 min with 100 μCi 111In-oxine sulphate and reinjected after dilution with the plasma. Mean labelling efficiency was 90%±3%, mean recovery 2 h after reinjection 76%±3% (mean±SD).
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Davies HH, Siegel BA, Mathias CA, Joist JH, Sherman LA, Welch MJ (1978) Scintigraphic detection of atherosclerotic lesions and venous thrombosis in man by indium-111-labelled autologous platelets. Lancet 1:1158–1187
Du Heyns AP, Badenhorst PN, Pieters H, Lötter MG, Minaar PC, Duyvene de Wit LJ, Van Reenen OR, Retief EP (1980) Preparation of a viable population of indium-111 labelled human blood platelets. Thromb Haemost 42:1473–1482
Fäger E, Widhalm K, Kaliman J, Höfer R (1982) Thrombozytenhalblebenszeit bei Patienten mit primärer Hyperlipoproteinämie vom Typ IIa, IIb und IV nach Fredrickson mit und ohne klinische Zeichen der Atherosklerose. Wien Klin Wochenschr 16:421–426
Hardeman M (1980) Autologous blood cell labelling using 111-indium-oxinate for in vivo investigations. Byk-Mallinckrodt Manual, p 12
Hawker RJ, Hawker LM, Wilkinson AR (1978) Use of indium-111 oxine to label human platelets. Lancet 2:483–492
Hawker RJ, Hawker LM (1979) Optimal method and labelling parameters of indium-111 human platelets. Thromb Haemost 42:18–29
Hawker RJ, Hawker LM, Wilkinson AR (1980) Indium (111-indium) labelled human platelets: Optimal method. Clin Sci 58:243–251
Reiter S, Angelberger P, Sinzinger H (1983) Human platelet labeling and viability testing after incubation with different tracers. 3rd International Austrian Atherosclerosis Conference, April 6 (in press)
Sinzinger H, Angelberger P, Kolbe H (1981) Influence of incubation time and temperature on In-111-oxine uptake by human platelets. Thromb Haemost 45:295
Thakur ML, Welch MJ, Joist JH, Coleman RE (1976) Indium-111-labelled platelets: studies on preparation and evaluation of in vitro and in vivo functions. Thromb Res 9:345–357
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sinzinger, H., Kolbe, H., Strobl-Jäger, E. et al. A simple and safe technique for sterile autologous platelet labelling using “Monovette” vials. Eur J Nucl Med 9, 320–322 (1984). https://doi.org/10.1007/BF00276462
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00276462