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Genetic studies on Rhizobiophage 16-3

I. Genes and functions on the chromosome

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Summary

A number of temperature-sensitive (ts) and lysis-deficient mutants were isolated from a bacteriophage of Rhizobium meliloti. The ts mutants were grouped by complementation. Functions were classified in relation to the eclipse and latent period. A genetic map of about 40 units was derived from crosses. The genes on the chromosome of the phage are arranged in the order of their presumed functions during the phage growth. They are located on the chromosome in sequence: immunity-early-late-lysis genes.

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Abbreviations

Ant :

altered antigene

C :

immunity gene

h :

host range

K :

velocity constant of antiphage serum

L :

lysis gene

m.o.i.:

multiplicity of infection

NTG:

N-methyl-N-nitro-N′nitrosoguanidine

P.F.U.:

plaque forming unit

Rh.41 :

Rhizobium meliloti strain 41

ti :

thermo-inducible

ts :

temperature-sensitive

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Authors and Affiliations

  1. Institute of Genetics, Biological Research Centre Hungarian Academy of Sciences, Szeged, Hungary

    L. Orosz, Z. Sváb, Á. Kondorosi & T. Sik

  2. Department of Genetics, Leeds University, LS2 9JT, Leeds, Yorkshire, England

    L. Orosz

Authors
  1. L. Orosz
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  2. Z. Sváb
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  3. Á. Kondorosi
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  4. T. Sik
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Communicated by F. Kaudewitz

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Orosz, L., Sváb, Z., Kondorosi, Á. et al. Genetic studies on Rhizobiophage 16-3. Molec. Gen. Genet. 125, 341–350 (1973). https://doi.org/10.1007/BF00276589

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  • DOI: https://doi.org/10.1007/BF00276589

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