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Electron microscopical observation of polyglucose synthesized from glucose-1-phosphate by the phosphorylase activity in rat skeletal muscles

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Summary

An improved demonstration of polyglucose synthesized histochemically from glucose-1-phosphate by the phosphorylase activity in rat skeletal muscle was made by use of electron histochemical techniques.

New polyglucose was found in the sarcoplasmic matrix among various organelles in muscle fibers embedded with epoxy resin. This polyglucose stained less densely with lead, forming amorphous aggregates or finer particulates with 100 to 150 Å in diameter, different from native glycogen which was separately observed in the same section.

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References

  • Eckner, F. A. O., Riebe, B. H., Moulder, P. V., Blackstone, E. H.: Polysaccharide synthesis in tissue sections; Evaluation of methods as an example of quality control in histochemistry. Histochemie 19, 340–354 (1969).

    Google Scholar 

  • Peace, D. C.: Histochemical techniques for electron microscopy, 2nd ed., p. 234. London: Acad. Press 1964.

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate a high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).

    Google Scholar 

  • Sasaki, M., Takeuchi, T.: Histochemical and electron microscopic observation of glycogen synthesized from glucose-1-phosphate by phosphorylase and branching enzyme in human muscle. J. Histochem. Cytochem. 11, 342–348 (1963).

    Google Scholar 

  • - - Ultracytochemical observations of glycogen intracellularly synthesized by the enzyme activities. Sixth Internat. Congr. for Elec. Micros. (Maruzen Co.), p. 77–78 (1966).

  • —: Electron histochemical observations of polysaccharide synthesized by enzyme activities in rat liver cells. Acta histochem. cytochem. 1, 197–209 (1968).

    Google Scholar 

  • Swanson, M. A.: Studies on the structure of polysaccharides. IV. Relation of the iodine color to the structure. J. biol. Chem. 172, 825–837 (1948).

    Google Scholar 

  • Takeuchi, T.: Histochemical demonstration of branching enzyme (amylo-1,4→1,6-transglucosidase) in animal tissues. J. Histochem. Cytochem. 6, 208–216 (1958).

    Google Scholar 

  • —: Histochemistry of intracellular polysaccharide synthesis. Ann. Histochem. 7, 61–80 (1962).

    Google Scholar 

  • —, Kuriaki, H.: Histochemical detection of phosphorylase in animal tissues. J. Histochem. Cytochem. 3, 153–160 (1955).

    Google Scholar 

  • —, Sasaki, M.: Histochemical and electron microscopic differences between native glycogen and polyglucose synthesized by phosphorylase in tissue cells. Acta histochem. cytochem. 1, 63–78 (1968).

    Google Scholar 

  • - - An electron histochemical observation of polysaccharide synthesized from uridine diphosphoglucose by the enzyme activity. Acta Histochem. Cytochem. 3 (1970).

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Takeuchi, T., Sasaki, M. Electron microscopical observation of polyglucose synthesized from glucose-1-phosphate by the phosphorylase activity in rat skeletal muscles. Histochemie 23, 310–318 (1970). https://doi.org/10.1007/BF00278361

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  • DOI: https://doi.org/10.1007/BF00278361

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