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Genetical and ultrastructural aspects of self and cross incompatibility in interspecific hybrids between self-compatible Lycopersicum esculentum and self-incompatible L. peruvianum

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Summary

Cytological and genetical analyses were made of the breeding system of embryo-cultured interspecific tomato hybrids between L. esculentum and L. peruvianum. It was found that fluorescence techniques and electron microscopy allowed a distinction to be made between pollen tubes inhibited by a unilateral incompatibility reaction and pollen tubes inhibited by a self-incompatibility reaction, after self-pollination of the hybrids or after reciprocal crossing between the hybrid and the parental species. The observed differences, if real and reliable, demonstrate that unilateral incompatibility in esculentum pollen tubes is governed by a single gametophytic factor which is either linked or allelic to the S-locus. This finding is discussed with reference to recent reports that unilateral incompatibility is controlled, in peruvianum styles, by a number of different dominant genes and it is concluded that these dominant genes, the S-locus of self-incompatibility and the gametophytic factor regulating the unilateral reaction in esculentum pollen belong to the same linkage group. The strong sterility barriers which prevent practically all backcrosses between the hybrid and the parental species were shown to be independent of the factors regulating stylar incompatibility. L. peruvianum is heterozygous for the sterility genes which prevent fertilization or embryo formation when the interspecific hybrid is crossed, as pistillate parent, to different accessions of L. peruvianum. One peruvianum stock was found which, as a pollinator, was highly cross-fertile with the hybrids.

The presence of a concentric endoplasmic reticulum in inhibited pollen tubes was observed to be a constant feature of both the self- and the unilateral incompatibility reactions and was interpreted as an indication that incompatibility might lead to a general cessation of protein synthesis. Although incompatible tubes very much resemble, in this respect, the pollen tubes cultured in vitro, it seems probable, on theoretical grounds, that the inhibition of pollen tubes in incompatible styles does not result from an absence of growth promoting substances but from the presence of a metabolic inhibitor.

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Authors and Affiliations

  1. Laboratorio Applicazioni Agricoltura del C.N.E.N., C.S.N., Casaccia, Roma, Italy

    D. De Nettancourt, M. Devreux & U. Laneri

  2. Services de Biologie, Communautés Européennes, 200, rue de la Loi, 1040, Bruxelles, Belgium

    D. De Nettancourt

  3. Laboratory of Electron Microscopy, Istituto di Botanica dell'Universitá di Siena, Italy

    M. Cresti, E. Pacini & G. Sarfatti

Authors
  1. D. De Nettancourt
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  2. M. Devreux
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  3. U. Laneri
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  4. M. Cresti
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  5. E. Pacini
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  6. G. Sarfatti
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Additional information

Communicated by H. F. Linskens

This article is contribution n ° 945 from the Biology Division of the European Communities and contribution n ° 387 from the Laboratorio Applicazioni Agricoltura del C.N.E.N. This work has been partly supported by a grant of the Italian National Research Council (C.N.R.) and by a sub-contract between the association EURATOM-ITAL and the CNEN.

Drs de Nettancourt and Devreux are members of the Biology Division of the European Communities; Dr. D. de Nettancourt is now at the Biology Division, European Communities, Bruxelles (Belgium).

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De Nettancourt, D., Devreux, M., Laneri, U. et al. Genetical and ultrastructural aspects of self and cross incompatibility in interspecific hybrids between self-compatible Lycopersicum esculentum and self-incompatible L. peruvianum . Theoret. Appl. Genetics 44, 278–288 (1974). https://doi.org/10.1007/BF00278744

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