Summary
We reported recently that intrasplenic transplantation of syngeneic pancreatic islets failed to cure obese-hyperglycaemic mice, despite a considerable growth of the grafted islets. In the present study, the role of sustained hyperglycaemia and hyperinsulinaemia for regeneration of transplanted islet cells was evaluated in these animals. Islets implanted into alloxan-diabetic C57BL/6J mice in numbers insufficient to restore normoglycaemia did not grow. There was, however, a statistically significant correlation between the mean volume of implanted islets and the degree of normalization of hyperglycaemia. Insulin treatment of these suboptimally islet-implanted C57BL/6J mice resulted in a volume increase of the implanted islets. When corresponding experiments were undertaken with alloxan-diabetic C57BL/KsJ mice, no effect was noted on hyperglycaemia and there was a drastic decrease in the volume of implanted islets. Islets implanted into old obese-hyperglycaemic mice that had returned to normoglycaemia but still were hyperinsulinaemic, decreased markedly in size. The present data suggest that neither hyperglycaemia nor hyperinsulinaemia per se are primarily responsible for the growth of islets in obese-hyperglycaemic mice. Furthermore, it is obvious that the genetic background is very important for morphological and functional responses of the islets to a prolonged period of hyperglycaemic stress.
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Andersson A, Eriksson U, Petersson B, Reibring L, Swenne I (1981) Failure of successful intrasplenic transplantation of islets from lean mice to cure obese-hyperglycaemic mice, despite islet growth. Diabetologia 20: 237–241
Strautz RL (1970) Studies of hereditary-obese mice (ob/ob) after implantation of pancreatic islets in millipore filter capsules. Diabetologia 6: 306–312
Gates RJ, Hunt MJ, Smith R, Lazarus NR (1972) Return to normal of blood-glucose, plasma-insulin, and weight gain in New Zealand obese mice after implantation of islets of Langerhans. Lancet 2: 250–253
Gates RJ, Hunt MJ, Lazarus NR (1974) Further studies on the amelioration of the characteristics of New Zealand obese (NZO) mice following implantation of islets of Langerhans. Diabetologia 10: 401–406
Swenne I, Bone AJ (1981) Effects, in tissue culture, of serum from obese mice on the DNA synthesis of the pancreatic B cell. Cell Biol Int Rep 5: 647–652
Westman S (1968) Development of the obese-hyperglycaemic syndrome in mice. Diabetologia 4: 141–149
Coleman DL (1978) Obese and diabetes: two mutant genes causing diabetes-obesity syndromes in mice. Diabetologia 14: 141–148
Howell SL, Taylor KW (1968) Potassium ions and the secretion of insulin by islets of Langerhans incubated in vitro. Biochem J 108: 17–24
Heding LG (1972) Determination of total serum insulin in insulintreated patients. Diabetologia 8: 260–266
Ostle B (1963) Statistics in research, 2nd ed. Ames, The Iowa State University Press
McEvoy RC, Hegre OD (1978) Syngeneic transplantation of fetal rat pancreas. II. Effect of insulin treatment on the growth and differentiation of pancreatic implants fifteen days after transplantation. Diabetes 27: 988–995
Brown J, Heininger D, Kuret J, Mullen Y (1981) Islet cell growth after transplantation of fetal pancreas and control of diabetes. Diabetes 30: 9–13
McEvoy RC (1981) Fetal rat pancreas in organ culture: Effect of exogenous insulin on the development of islet cells. Horm Metab Res 13: 5–8
Rabinovitch A, Quigley C, Russell T, Patel Y, Mintz DH (1982) Insulin and multiplication stimulating activity (an insulin-like growth factor) stimulate islet B cell replication in neonatal rat pancreatic monolayer cultures. Diabetes 31: 160–164
Coleman DL, Hummel KP (1967) Studies with the mutation, diabetes, in the mouse. Diabetologia 3: 238–248
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Andersson, A. The influence of hyperglycaemia, hyperinsulinaemia and genetic background on the fate of intrasplenically implanted mouse islets. Diabetologia 25, 269–272 (1983). https://doi.org/10.1007/BF00279942
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DOI: https://doi.org/10.1007/BF00279942