Summary
Mitotic cultures synchronised either by a feed-starve protocol or by elutriation have been used to show that the Saccharomyces cerevisiae DNA primase I gene is periodically expressed in the cell cycle. The transcript increases many-fold in late G1 and reaches a peak at the same time as four other genes essential for DNA synthesis, CDC8, CDC9, CDC21 and POL1. The primase I transcript is also regulated in meiosis, reaching maximal levels during premeiotic DNA synthesis.
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Communicated by W. Gajewski
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Johnston, L.H., White, J.H.M., Johnson, A.L. et al. Expression of the yeast DNA primase gene, PRI1, is regulated within the mitotic cell cycle and in meiosis. Molec. Gen. Genet. 221, 44–48 (1990). https://doi.org/10.1007/BF00280366
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DOI: https://doi.org/10.1007/BF00280366