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The arrangement of microtubules and the attachment of chromosomes to the spindle during anaphase in tipulid spermatocytes

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Abstract

Analysis of serial sections oriented parallel to the interpolar spindle axis revealed the following results. Autosomes in anaphase of the 1. meiotic division of Pales ferruginea spermatocytes are attached to the spindle in two ways: 1. The short kinetochoric microtubules (kMTs) diverge and interdigitate with the axial mass of non-kinetochoric microtubules (nkMTs). 2. The chromosome surface shows projections which protrude between the mass of nkMTs. — At the level of anaphase plates the concentration of nkMTs is higher than in the interzone. — The lagging sex chromosomes at the equator become stretched by anaphase forces during autosomal movement. — The mean length of nkMTs in metaphase is 3.0±0.1 μm, in anaphase 2.6±0.1 μm, possibly indicating an overall MT shortening in anaphase. Spindle architecture and aspects of anaphase forces are discussed.

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  1. Max-Planck-Institut für Zellbiologie, Tübingen

    Harald Fuge

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  1. Harald Fuge
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Fuge, H. The arrangement of microtubules and the attachment of chromosomes to the spindle during anaphase in tipulid spermatocytes. Chromosoma 45, 245–260 (1974). https://doi.org/10.1007/BF00283409

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  • DOI: https://doi.org/10.1007/BF00283409

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