Abstract
All subspecies of black rats,(Rattus rattus) used in the present study are characterized by having large and clear C-bands at the centromeric region. The appearance of the bands, however, is different in the subspecies. Chromosome pair No. 1 in Asian type black rats (2n=42), which are characterized by an acrocentric and subtelocentric polymorphism, showed C-band polymorphism. In Philippine rats,(R. rattus mindanensis) the pair was subtelocentric with C-bands, but in Malayan black rats,(R. rattus diardii) it was usually acrocentric with C-bands. In Hong-Kong,(R. rattus flavipectus) and Japanese black rats,(R. rattus tanezumi) it was polymorphic with respect to the presence of acrocentrics with C-bands or subtelocentrics without C-bands. The other chromosome pairs showed clear C-bands, but in Hong-Kong black rats the pairs No. 2 and 5 were polymorphic with and without C-bands. In Japanese black rats, 6 chromosome pairs (No. 3, 4, 7, 9, 11 and 13) were polymorphic in regard to presence and absence of C-bands, but the other 5 chromosome pairs (No. 2, 5, 6, 8 and 10) showed always absence of C-bands. Only pair No. 12 usually showed C-bands. C-bands in small metacentric pairs (No. 14 to 20) in Asian type black rats were generally large in size, but those in the Oceanian (2n=38) and Ceylon type black rats (2n=40) were small. In the hybrids between Asian and Oceanian type rats, heteromorphic C-bands, one large and the other small, were observed. Based on the consideration of karyotype evolution in the black rats, the C-band is suggested to have a tendency toward the diminution as far as the related species are concerned.
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References
Arrighi, F.E., Hsu, T.C.: Location of heterochromatin in human chromosomes. Cytogenetics 10, 81–86 (1971)
Brown, J.E., Jones, K.W.: Localization of satellite DNA in the microchromosomes of the Japanese quail by in situ hybridization. Chromosoma (Berl.) 38, 313–318 (1972)
Chen, T.R., Ruddle, F.H.: Karyotype analysis utilizing differentially stained constitutive heterochromatin of human and murine chromosomes. Chromosoma (Berl.) 34, 51–72 (1972)
Comings, D.E., Mattoccia, E.: DNA of mammalian and avian heterochromatin. Exp. Cell Res. 71, 113–131 (1972)
Craig-Holmes, A.P., Moore, F.B., Shaw, M.W.: Polymorphism of human C-band heterochromatin. I. Frequency of variants. Amer. J. hum. Genet. 25, 181–192 (1973)
Craig-Holmes, A.P., Shaw, M.W.: Polymorphism of human constitutive heterochromatin. Science 174, 702–704 (1971)
Forejt, J.: Centromeric heterochromatin polymorphism in the house mouse. Chromosoma (Berl.) 43, 187–201 (1973)
Hoehn, H., Martin, G.M.: Heritable alteration of human constitutive heterochromatin induced by mitomycin C. Exp. Cell Res. 75, 275–278 (1972)
Hsu, T.C., Cooper, J.E.K., Mace, M.L., Brinkley, B.R.: Arrangement of centromeres in mouse cells. Chromosoma (Berl.) 34, 73–87 (1971)
Jones, K.W.: Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature (Lond.) 225, 912–915 (1970)
Jones, K.W., Corneo, G.: Location of satellite and homogenous DNA sequences on human chromosomes. Nature (Lond.) New Biol. 233, 268–271 (1971)
Kato, H., Tsuchiya, K., Yosida, T.H.: Constitutive heterochromatin of Indian muntjac chromosomes revealed by DNase treatment and a C-banding technique. Canad. J. Genet. Cytol. 16, 273–280 (1974)
Pardue, M.L., Gall, J.G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970)
Polani, P.E.: Centromere location at meiosis and the position of chiasmata in male and female mouse. Chromosoma (Berl.) 36, 342–374 (1972)
Raman, R., Sharma, T.: DNA replication, G-and C-bands and meiotic behaviour of supernumerary chromosomes of Rattus rattus (Linn.). Chromosoma (Berl.) 45, 111–119 (1974)
Saunders, G.F., Hsu, T.C., Getz, M.L., Simes, E.L., Arrighi, F.E.: Location of human satellite DNA in human chromosomes. Nature (Lond.) New Biol. 236, 244–246 (1972)
Saunders, G.F., Shirakawa, S., Saunders, P.P., Arrighi, F.E., Hsu, T.C.: Populations of repeated DNA sequences in the human genome. J. molec. Biol. 63, 323–334 (1972)
Sumner, A.T.: A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75, 304–306 (1972)
Yosida, T.H., Kato, H., Tsuchiya, K., Sagai, T., Moriwaki, K.: Cytogenetical survey of black rats, Rattus rattus, in Southwest and Central Asia, with special regard to the evolutional relationship between three geographical types. Chromosoma (Berl.) 45, 99–109 (1974)
Yosida, T.H., Moriwaki, K., Sagai, T.: Oceanian type black rats (Rattus rattus) with a subtelocentric M2 chromosome and C-type transferrin obtained from North America. Experientia (Basel) 30, 742–744 (1974)
Yosida, T.H., Nakamura, A., Fukaya, T.: Chromosomal polymorphism in Rattus rattus (L.) collected in Kusudomari and Misima. Chromosoma (Berl.) 16, 70–78 (1965)
Yosida, T.H., Sagai, T.: Banding pattern analysis of polymorphic karyotypes in in the black rat by a new differential staining technique. Chromosoma (Berl.) 37, 287–294 (1972)
Yosida, T.H., Sagai, T.: Similarity of Giemsa banding patterns of chromosomes in several species of the genus Rattus. Chromosoma (Berl.) 41, 93–101 (1973)
Yosida, T.H., Tsuchiya, K., Imai, H., Moriwaki, K.: New chromosome types of black rat, Rattus rattus, collected in Oceania and hybrid between Japanese and Australian rats. Jap. J. Genet. 44, 89–91 (1969)
Yosida, T.H., Tsuchiya, K., Moriwaki, K.: Karyotypic differences of black rats, Rattus rattus, collected in various locatities of East and Southeast Asia and Oceania. Chromosoma (Berl.) 33, 252–267 (1971a)
Yosida, T.H., Tsuchiya, K., Moriwaki, K.: Karyotype and serum transferrin patterns of hybrids between Asian and Oceanian black rats, Rattus rattus. Chromosoma (Berl.) 34, 40–50 (1971b)
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Yosida, T.H., Sagai, T. Variation of C-bands in the chromosomes of several subspecies of Rattus rattus . Chromosoma 50, 283–300 (1975). https://doi.org/10.1007/BF00283472
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DOI: https://doi.org/10.1007/BF00283472