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Cytological mapping of the genes assigned to the human A 1 chromosome by use of radiation-induced chromosome breakage in a human-Chinese hamster hybrid cell line

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Summary

A human-Chinese hamster hybrid cell line was treated with X-rays and subsequently cloned. In two clones, segregation between three markers assigned to the human A1 chromosome (6-phosphogluconate dehydrogenase, phosphoglucomutase 1 and peptidase C) was found. Chromosome analysis revealed an aberrated human A1 chromosome in both clones. The results suggest the following gene sequence on chromosome A1: 6 PGD, PGM1 (on the short arm), Pep C (on the long arm).

Zusammenfassung

Eine Zell-Linie (Hybride zwischen Zellen vom Menschen und vom Chinesischen Hamster) wurde mit Röntgenstrahlen behandelt und anschließend kloniert. In zwei Klonen wurde eine Aufspaltung bei drei Markern beobachtet, die zum menschlichen A1-Chromosom gehören (6-Phosphogluconat-Dehydrogenase; Phosphoglucomutase 1; Peptidase C). Die Chromosomenanalyse zeigte ein aberrantes menschliches A1-Chromosom in beiden Klonen. Die Ergebnisse legen die folgende Reihenfolge nahe: 6 PGD; PGM1 (auf dem kurzen Arm); Pep C (auf dem langen Arm).

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, Erasmus University, P. O. Box 1738, Rotterdam, The Netherlands

    Wim Burgerhout & Dirk Bootsma

  2. Medical Biological Laboratory TNO, State University Leiden, c/o P. O. Box 45, Rijswijk, The Netherlands

    Harry van Someren

  3. Laboratory for Molecular Genetics, State University Leiden, c/o P. O. Box 45, Rijswijk, The Netherlands

    Harry van Someren

Authors
  1. Wim Burgerhout
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  2. Harry van Someren
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  3. Dirk Bootsma
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Burgerhout, W., van Someren, H. & Bootsma, D. Cytological mapping of the genes assigned to the human A 1 chromosome by use of radiation-induced chromosome breakage in a human-Chinese hamster hybrid cell line. Humangenetik 20, 159–162 (1973). https://doi.org/10.1007/BF00284852

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  • DOI: https://doi.org/10.1007/BF00284852

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