Abstract
The correct positioning of the plane of the cell plate of the dividing guard mother cell (GMC) of Allium is controlled by reorientation movements of the spindle-phragmoplast structure during anaphase-telophase, as described in a previous publication (Palevitz and Hepler, 1974). In an attempt to clarify the mechanism of movement a series of experiments involving the application of different drugs has been performed. The metabolic inhibitors, azide, 2–4 DNP and cyanide reversibly block reorientation. The antimicrotubule agents, colchicine, vinblastine sulfate and isopropyl-N-phenylcarbamate (IPC) prevent reorientation and cause shrinkage of the interzone. They also produce aberrant and malaligned cell plates. Caffeine has no effect on reorientation although it inhibits cell plate formation. Cytochalasin B (CB) produces variable effects; frequently, but not always, it blocks reorientation and induces misshapened cell plates. These results suggest that determination of the correct plane of division is an energy requiring process. In addition interzone microtubules appear essential for spindle reorientation either by acting as cytoskeletal agents or by providing the force necessary for movement.
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Palevitz, B.A., Hepler, P.K. The control of the plane of division during stomatal differentiation in Allium . Chromosoma 46, 327–341 (1974). https://doi.org/10.1007/BF00284885
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DOI: https://doi.org/10.1007/BF00284885