Skip to main content
SpringerLink
Log in

Fast uptake kinetics in vitro of 51Cr (VI) by red blood cells of man and rat

  • Original Investigations
  • Published:
Archives of Toxicology Aims and scope Submit manuscript

Abstract

Fast transport kinetics of 51Cr (VI) into red blood cells (RBCs) in vitro were studied. No significant species differences were found between RBCs of man and rat. The uptake of 51Cr (VI) by RBCs in whole blood was composed of two different first order processes of different velocities (apparent t1/2 of 22.7 s and 10.4 min for man and 6.9 s and 10.1 min for rat, respectively). However, even after longer time periods a fixed portion of approximately 15% of the administered dose remained in the plasma and did not penetrate into RBCs Over the entire concentration range studied (10 μM–50 mM), the fast initial uptake followed Michaelis-Menten kinetics. The maximal capacity of this Cr(VI) transport into RBCs of man and rat was 3.1×108 CrO4 2− ions × cell−1 × min−1 and 2.5×108 CrO4 −2 ions × cell−1 × min−1, respectively. It is likely that Cr(VI) is transported into RBCs via a physiological anion carrier (“band-3-protein”).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bianchi V, Celotti L, Lanfranchi F, Majone F, Marin G, Montaldi A, Sponza G, Tamino G, Venier P, Zentedeschi A, Levis AG (1983) Genetic effects of chromium compounds. Mutat Res 117: 279–300

    Google Scholar 

  • Dalmark M (1975) Chloride transport in human red cells. J Physiol 250: 39–64

    Google Scholar 

  • Deuticke B (1967) Über die Kinetik der Phosphat-Permeation in den Menschen-Erythrozyten bei Variation von extrazellulärer Phosphat-Konzentration, Anionen-Milieu und Zell-Volumen. Pflügers Arch 296: 21–38

    Google Scholar 

  • Gray SJ, Sterling K (1950) The tagging of red cells and plasma proteins, with radioactive chromium. J Clin Invest 29: 1604–1613

    Google Scholar 

  • Hayes RB (1982) Carcinogenic effects of chromium In: Langard S (ed) Biological and environmental aspects of chromium. Elsevier Biomedical Press, Amsterdam, pp 221–247

    Google Scholar 

  • Hladky SB, Rink TL (1977) pH-Equilibrium across the red cell membrane. In: Ellory JC, Lew VL (eds) Membrane transport in red cells. Academic Press, London, pp 115–135

    Google Scholar 

  • ICHS (1973) Standard techniques for the measurement of red-cell and plasma volume. Brit J Haem 25: 801–814

    Google Scholar 

  • Korallus U, Harzdorf C, Lewalter J (1984) Experimental basis for ascorbic acid therapy of poisoning by hexavalent chromium compounds. Int Arch Occup Environ Health 53: 247–256

    Google Scholar 

  • Langard S, Vigander T (1983) Occurence of lung cancer in workers producing chromium pigments. J Ind Med 40: 71–74

    Google Scholar 

  • Ormos G, Manyai S (1977) Chemical modification of erythrocytes. Effect on the velocity of chromate uptake. Acta Biochim Biophys Acad Sci Hung 12: 343–352

    Google Scholar 

  • Prins HK (1962) The binding of 51chromium by human erythrocytes. Vox Sang 7: 370–372

    Google Scholar 

  • Rollinson CL, Rosenbloom E, Lindsay J (1967) Reactions of chromium (III) with biological substances. In: Proceedings of the 7th International Congress of Nutrition, Hamburg 1966, Vol 5, Pergamon Press, Oxford, pp 692–697

    Google Scholar 

  • Rothstein A, Cabantchik ZI, Knauf P (1976) Mechanism of anion transport in red blood cells: Role of membrane proteins. Fed Proc 35: 3–10

    Google Scholar 

  • Samitz MH, Scheiner DM, Katz S (1968) Ascorbic acid in the prevention of chrome dermatitis. Arch Environ Health 17: 44–45

    Google Scholar 

  • Schnell KF, Gerhardt S, Schöppe-Fredenburg A (1977) Kinetic characteristics of the sulfate self-exchange in human red blood, cells and red blood cell ghosts. Membr Biol 30: 319–350

    Google Scholar 

  • Sunderman F (1984) Recent advances in metal carcinogenesis. Ann Clin Lab Sci 14: 93–122

    Google Scholar 

  • Tkeshelashvili LK, Shearman CW, Zakour RA, Koplitz RM, Loeb LA (1980) Effects of arsenic, selenium and chromium on the fidelity of DNA synthesis. Cancer Res 40: 2455–2460

    Google Scholar 

  • Tsapakos MJ, Wtterhahn KE (1983) The interaction of chromium on nucleic acids. Chem Biol Interact 46: 265–277

    Google Scholar 

  • Wiegand HJ, Ottenwälder H, Bolt HM (1984) Toxicol Lett 22: 273–276

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Arbeitsphysiologie an der Universität Dortmund, Ardeystr. 67, D-4600, Dortmund 1, Federal Republic of Germany

    H. J. Wiegand, H. Ottenwälder & H. M. Bolt

Authors
  1. H. J. Wiegand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Ottenwälder
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. M. Bolt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wiegand, H.J., Ottenwälder, H. & Bolt, H.M. Fast uptake kinetics in vitro of 51Cr (VI) by red blood cells of man and rat. Arch Toxicol 57, 31–34 (1985). https://doi.org/10.1007/BF00286571

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00286571

Key words

Search

Navigation