Abstract
Male hybrids of the cross D. azteca x D. athabasca are larger (hybrid giant males) than their parents, whereas hybrid females are of the same size as the parental species. Microspectrophotometric measurements have shown that the larval polytene salivary gland chromosomes of hybrid giant males undergo one more endoreplication than those of their sisters or parents. Replication patterns of the larval salivary gland chromosomes were compared after pulse labeling with 3H-thymidine and autoradiography. In females of either species as well as of hybrids X-chromosomes and autosomes are equally labeled, i.e. all chromosome arms replicate synchronously. In males, however, often fewer sites are labeled on the X-chromosome than on the autosomes. In addition, in a significant number of nuclei from D. athabasca males and also from hybrid giant males the converse can also be observed: i.e. more sites are labeled on the X-chromosome than on the autosomes. The modified labeling patterns are interpreted as an indication of a time-shift in the replication of hemizygous X-chromosomes in males, in relation to the autosomes.
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Arcos-Terán, L.: DNS-Replication und die Natur der spät replizierenden Orte im X-Chromosom von D. melanogaster. Chromosoma (Berl.) 37, 233–296 (1972)
Bauer, H., Dobzhansky, Th.: A comparison of gene arrangement in D. azteca and D. athabasca. Genetics 22, 185 (1937)
Beermann, W.: Riesenchromosomen. Protoplasmatologia VI/D. Wien: Springer 1962
Berendes, H.D.: Differential replication of male and female X-chromosomes in Drosophila. Chromosoma (Berl.) 20, 32–43 (1966)
Bridges, C.B.: The origin of variation in sexual and sexlimited characters. Amer. Naturalist 56, 51–63 (1922)
Cohen, E., Gotchel, B.V.: Histones of polytene and non-polytene nuclei of Drosophila melanogaster. J. biol. Chem. 246, 1841–1848 (1971)
Dobzhansky, Th.: The X-chromosome in the larval salivary glands of hybrids in D. insularis x D. tropicalis. Chromosoma (Berl.) 8, 691–698 (1957)
Dobzhansky, Th., Socolov, S.: Structure and variation of the chromosomes in Drosophila azteca. J. Hered. 30, 3–19 (1939)
Grabusewycz-Garcia, N.: Cytological and autoradiographic studies in Sciara coprophila salivary gland chromosomes. Chromosoma (Berl.) 15, 312–344 (1964)
Hägele, K.: DNS-Replikationsmuster der Speicheldrüsenchromosomen von Chironomiden. Chromosoma (Berl.) 31, 91–138 (1970)
Hägele, K.: Komplementäre DNS-Replikationsmuster bei Drosophila melanogaster. Chromosoma (Berl.) 41, 231–236 (1973)
Hägele, K., Kalisch, W.E.: Initial phase of DNA synthesis in Drosophila melanogaster. Chromosoma (Berl.) 47, 403–413 (1974)
Kasten, F.H.: The chemistry of Schiff's reagent. Int. Rev. Cytol. 10, 1–100 (1960)
Keyl, H.G.: Duplikationen von Untereinheiten der chromosomalen DNS während der Evolution von Chironomus thummi. Chromosoma (Berl.) 17, 139–180 (1965)
Korge, G.: Dosiskompensation und Dosiseffekt für RNS-Synthese in Chromosomen-Puffs von D. melanogaster. Chromosoma (Berl.) 30, 430–464 (1970)
Lakhotia, S.C., Mukherjee, A.S.: Chromosomal basis of dosage compensation in Drosophila. III. Early completion of replication by the polytene X-chromosome in male: Further evidence and its implications. J. Cell Biol. 47, 18–33 (1970)
Ledebur-Villiger, M. v.: Cytology and nucleic acid synthesis of parthenogenetically activated sea urchin eggs. Exp. Cell Res. 72, 285–308 (1972)
Lucchesi, I.C.: Dosage compensation in Drosophila. Ann. Rev. Genetics 7, 225–237 (1973)
Miller, D.D., Voelker, R.A.: Salivary gland chromosome variation in the affinis subgroup. I. The C-chromosome of “western” and “eastern” D. athabasca. J. Hered. 59, 87–98 (1968)
Miller, D.D., Voelker, R.A.: Salivary gland chromosome variation in the affinis subgroup. III. The long arm of the X-chromosome in “western” and “eastern” D. athabasca. J. Hered. 60, 231–238 (1969)
Miller, D.D., Voelker, R.A.: Salivary gland chromosome variation in the affinis subgroup. IV. The short arm of the X-chromosome in “western” and “eastern” Drosophila athabasca. J. Hered. 60, 306–311 (1969)
Miller, D.D., Voelker, R.A.: Salivary gland chromosome variation in the affinis subgroup V. The B and E chromosomes of “western” and “eastern” Drosophila athabasca. J. Hered. 63, 2–10 (1972)
Mukherjee, A.S., Beermann, W.: Synthesis of ribonucleic acid by X-chromosomes of D. melanogaster and the problem of dosage compensation. Nature (Lond.) 207, 785–786 (1965)
Mukherjee, A.S., Chatterjee, S.N.: Chromosomal basis of dosage compensation in Drosophila. VIII. Faster Replication and hyperactivity of both arms of the X-chromosome in males of Drosophila pseudoobscura and their possible significance. Chromosoma (Berl.) 53, 91–105 (1975)
Novitski, E.: Chromosome variation in D. athabasca. Genetics 31, 508–524 (1946)
Offermann, C.A.: Branched chromosomes as symmetrical duplications. J. Genet. 32, 103–116 (1936)
Patterson, J.T., Stone, W.S.: Evolution on the genus Drosophila. New York: The Macmillan Company 1952
Rasch, E.M., Barr, H.J., Rasch, R.W.: The DNA content of sperm of Drosophila melanogaster. Chromosoma (Berl.) 33, 1–18 (1971)
Rodmann, T.C.: Relationship of developmental stage to initiation of replication in polytene nuclei. Chromosoma (Berl.) 23, 271–287 (1968)
Rudkin, G.T.: Replication in polytene chromosomes. In: Developmental studies on giant chromosomes (results and problems in cell differentiation) Vol. 4, (W. Beermann, ed.), p. 58–85. Berlin-Heidelberg-New York: Springer 1972
Speiser, Ch.: Quantitative DNS-Bestimmung im Verlauf der Ontogenese von Acricotopus lucidus (Chironomide). Dissertation, Universität Hohenheim (1973)
Sturtevant, A.H.: The classification of the genus Drosophila with description of nine new species. Univ. Texas Publ. 4213, 5–51 (1942)
Sturtevant, A.H., Dobzhansky, Th.: Observations on the species related to new forms of D. affinis, with description of seven. Amer. Naturalist 70, 537–584 (1936)
Tiepolo, L., Laudani, U.: DNA-Synthesis in polytenic chromosomes of Anopheles atroparvus. Chromosoma (Berl.) 36, 305–312 (1972)
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Part of this work has been communicated at the German-Soviet Symposium about Genome Organization and Function, München, FRG, 1976
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Meer, B. Anomalous development and differential DNA replication in the X-Chromosome of a Drosophila hybrid. Chromosoma 57, 235–260 (1976). https://doi.org/10.1007/BF00295210
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DOI: https://doi.org/10.1007/BF00295210