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Translation of immunoglobulin mRNAs in a wheat germ cell-free system

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Abstract

An unfractionated wheat germ cell-free system will efficiently translate immunoglobulin messenger RNAs from four murine myelomas. The system responds as well to immunoglobulin mRNA as to globin mRNA and translates mRNAs for both heavy and light immunoglobulin chains. The mRNAs for both kappa and lambda chains are translated into polypeptides 1700–2000 daltons larger than the authentic light chains. Chain completion is poor with most mRNAs, but improves when the reactions are done at KCl concentrations considerably higher than the optimum for maximal incorporation of radioactivity. Mammalian transfer RNA stimulates translation of all mRNAs tested.

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References

  1. Efron, D. and Marcus, A., FEBS Letters 33, 23 (1973).

    Google Scholar 

  2. Roberts, B. E. and Paterson, B. M., Proc. Nat. Acad. Sci. U.S. 70, 2330 (1973).

    Google Scholar 

  3. Stavnezer, J. and Huang, R. C. C., Nature New Biology 230, 172 (1971).

    Google Scholar 

  4. Swan, D., Aviv, H., and Leder, P., Proc. Nat. Acad. Sci. U.S. 69, 1967 (1972).

    Google Scholar 

  5. Milstein, C., Brownlee, G. G., Harrison, T. M., and Mathews, M. B., Nature New Biology 239, 117 (1972).

    Google Scholar 

  6. Mach, B., Faust, C., and Vassalli, P., Proc. Nat. Acad. Sci. U.S. 70, 451 (1973).

    Google Scholar 

  7. Schechter, I., Proc. Nat. Acad. Sci. U.S. 70, 2256 (1973).

    Google Scholar 

  8. Cowan, N. J. and Milstein, C., Eur. J. Biochem. 36, 1 (1973).

    Google Scholar 

  9. Potter, M., Dreyer, W. J., Kuff, E. L., and McIntire, K. R., J. Mol. Biol. 8, 814 (1964).

    Google Scholar 

  10. Dugan, E. S., Bradshaw, R. A., Simms, E. S., and Eisen, H. N., Biochemistry 12, 5400 (1973).

    Google Scholar 

  11. Horibata, K. and Harris, A. W., Exptl. Cell. Res. 60, 61 (1970).

    Google Scholar 

  12. Schreier, M. H. and Staehelin, T., J. Mol. Biol. 73, 329 (1973).

    Google Scholar 

  13. Aviv, H., Boime, I., and Leder, P., Proc. Nat. Acad. Sci. U.S. 68, 2303 (1971).

    Google Scholar 

  14. Bollum, F. J., Methods in Enzymology 12B, 169 (1968).

    Google Scholar 

  15. Fairbanks, G.Jr., Levinthal, C., and Reeder, R. H., Biochem. Biophys. Res. Commun. 20, 393 (1965).

    Google Scholar 

  16. Laemmli, U. K., Nature 227, 680 (1970).

    Google Scholar 

  17. Gray, W. R., Dreyer, W. J., and Hood, L., Science 155, 465 (1967).

    Google Scholar 

  18. Svasti, J. and Milstein, C., Biochem. J. 128, 427 (1972).

    Google Scholar 

  19. Dayhoff, M. O., Hunt, L. T., McLaughlin, P. J., and Barker, W. C., in M. O.Dayhoff (ed.), Atlas of Protein Sequence and Structure, National Biomedical Research Foundation, Washington, 1972, Vol. 5, pp D-59, D-70.

    Google Scholar 

  20. Metafora, S., Terada, M., Dow, L. W., Marks, P. A., and Bank, A., Proc. Nat. Acad. Sci. U.S. 69, 1299 (1972).

    Google Scholar 

  21. Underdown, B. J., Simms, E. S., and Eisen, H. N., Biochemistry 10, 4395 (1971).

    Google Scholar 

  22. Schubert, D., J. Mol. Biol. 51, 287 (1970).

    Google Scholar 

  23. Jacobs-Lorena, M. and Baglioni, C., Biochemistry 11, 4970 (1972).

    Google Scholar 

Download references

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Authors and Affiliations

  1. Molecular Biology Laboratory, Biochemistry and Biophysics Unit, The Walter and Eliza Hall Institute of Medical Research, 3050, Melbourne, Victoria, Australia

    B. J. Schmeckpeper, Suzanne Cory & J. M. Adams

Authors
  1. B. J. Schmeckpeper
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  2. Suzanne Cory
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  3. J. M. Adams
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Publication number 2004 from The Waler and Eliza Hall Institute.

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Schmeckpeper, B.J., Cory, S. & Adams, J.M. Translation of immunoglobulin mRNAs in a wheat germ cell-free system. Mol Biol Rep 1, 355–363 (1974). https://doi.org/10.1007/BF00309570

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  • DOI: https://doi.org/10.1007/BF00309570

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