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Cytochemical significance of acridine orange staining of human plasmodia with some comments on double-stranded DNA

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Summary

Air-dried blood smears and erythrocyte suspension from patients infected with Plasmodium falciparum, stained under optimal conditions with acridine orange, permit easy detection of plasmodia with fluorescence microscopy together with a clear cytochemical colour differentiation of nuclear DNA (green or green-yellow) and cytoplasmic RNA (orange-red fluorescence). Judging from fluorescence characteristics of nuclei (DNase sensitive and RNase resistant green or green-yellow), the plasmodial DNA appears to be double-stranded.

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  1. Division of Microbiology, Central Drug Research Institute, Lucknow, India

    G. P. Dutta

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  1. G. P. Dutta
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Dutta, G.P. Cytochemical significance of acridine orange staining of human plasmodia with some comments on double-stranded DNA. Histochemie 24, 29–32 (1970). https://doi.org/10.1007/BF00310000

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  • DOI: https://doi.org/10.1007/BF00310000

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