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Chromosomal proteins in hepatocarcinogenesis

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Summary

The chromosomal proteins of rat liver were studied by SDS-gel electrophoresis during the process of nitrosomorpholine-induced hepatocarcinogenesis, in the primary hepatomas thus obtained, and in their metastases. It was found that an increased proteolytic activity was present in liver homogenates from carcinogen-fed animals which caused differences between the nonhistone chromosomal proteins of control and carcinogen-treated livers. These differences disappeared in the presence of the protease inhibitor PMSF.

In the primary hepatomas slight quantitative changes were observed: an increased amount of two proteins of 43000 and 63000 daltons molecular weight, respectively, and a decrease in the histone subfraction H 1°. In the metastases both quantitative and qualitative differences were detected: a strong decrease in the protein bands corresponding to the contractile proteins α-tubulin, β-tubulin, and actin; an increased content of the 63000 dalton protein; the appearance of new proteins of approximately 60000, 90000, and 120000 daltons molecular weight, and the complete disappearance of histone H1°.

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Authors and Affiliations

  1. Institute of Biochemistry, Bulgarian Academy of Sciences, Sofia

    R. Tsanev & D. Hadjiolov

  2. Institute of Oncology, Medical Academy, Sofia, Bulgaria

    R. Tsanev & D. Hadjiolov

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  1. R. Tsanev
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  2. D. Hadjiolov
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Tsanev, R., Hadjiolov, D. Chromosomal proteins in hepatocarcinogenesis. Z. Krebsforsch. 91, 237–247 (1978). https://doi.org/10.1007/BF00312286

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  • DOI: https://doi.org/10.1007/BF00312286

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