Skip to main content
SpringerLink
Log in

Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD)

  • Original Paper
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

One of the most important rice pathogens is Fusarium moniliforme (perfect stage: Gibberella fujikuroi), the causal agent of the super-elongation (“bakanae”) disease. Thirty-seven strains of this species from different geographical regions were analyzed for their ability to produce gibberellins (GA) and for genetic relatedness by random amplified polymorphic DNA (RAPD). All GA-producing isolates showed nearly identical RAPD patterns using 51 oligonucleotide nona- and deca-mers as arbitrary primers. On the other hand, large differences between GA-nonproducing isolates were obtained. Comparison of the RAPD patterns with those of the tester strains of the six known mating populations (A,B,C,D,E,F) of G. fujikuroi showed that all producer strains belong to mating population C and all nonproducer isolates to other mating populations. Evidence for the usefulness of the RAPD technique to distinguish between mating populations was provided by sexual crossings. Consensus phylogenetic trees based on RAPDs were constructed by the Phylogenetic Analysis Using Parsimony (PAUP) system. In combination with morphological analysis, RAPD can distinguish between different species of the genus Fusarium. These investigations may find an application in the diagnosis of unknown Fusarium spp. and in distinguishing isolates of G. fujikuroi within the section Liseola.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arnau J, Housego AP, Oliver RP (1994) The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum. Curr Genet 25: 438–444

    Google Scholar 

  • Aufauvre-Brown A, Cohen J, Holden DW (1992) Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus funigatus. J Clin Microbiol 30: 2991–2993

    Google Scholar 

  • Brückner B, Blechschmidt D, Schubert B (1989) Fusarium moniliforme Sheld. — a fungus producing a broad spectrum of bioactive metabolites. Zentralbl Mikrobiol 144: 3–12

    Google Scholar 

  • Büttner P, Koch F, Voigt K, Quidde T, Risch S Blaich R, Brückner B, Tudzynski P (1994) Variations in ploidy among isolates of Botrytis cinerea: implications for genetic and molecular analyses. Curr Genet 25: 445–450

    Google Scholar 

  • Coddington A, Matthews PM, Cullis C, Smith KH (1987) Restriction-digest patterns of total DNA from different races of Fusarium oxysporum f. sp. pisi — an improved method for race classification. J Phytopathol 118: 9–20

    Google Scholar 

  • Cenis JL (1993) Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res 20: 2380

    Google Scholar 

  • Correll JC, Gordon TR, McCain AH (1992) Genetic diversity in California and Florida populations of the pitch canker fungus Fusarium subglutinans f. sp. pini. Phytopathology 82: 415–420

    Google Scholar 

  • Cisar CR, Spiegel FW, TeBeest DO, Trout C (1994) Evidence for mating between isolates of Colletotrichum gloeosporioides with different host specificities. Curr Genet 25: 330–335

    Google Scholar 

  • Crowhurst RN, Hawthorne BT, Rikkerink EHA, Templeton MD (1991) Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA. Curr Genet 20: 391–396

    Google Scholar 

  • Dickman MB, Partidge JE (1989) Use of molecular markers for monitoring fungi involved in stalk rot of corn. Theor Appl Genet 77: 535–539

    Google Scholar 

  • Durand N, Reymond P, Fevre M (1993) Randomly amplified polymorphic DNAs assess recombination following an induced parasexual cycle in Penicillium roqueforti. Curr Genet 24: 417–420

    Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13

    Google Scholar 

  • Feinberg AP, Vogelstein (1984) Addendum. Anal Biochem 137: 266–267

    Google Scholar 

  • Fekete C, Nagy R, Debets AJM, Hornok L (1993) Electrophoretic karyotypes and gene mapping in eight species of the Fusarium sections Arthrosporiella and Sporotrichiella. Curr Genet 24: 500–504

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791

    Google Scholar 

  • Förster H, Coffey MD (1992) Molecular characterization of Phytophthora isolates with non-papillate sporangia causing root rot of raspberry using mtDNA restriction fragment length polymorphisms. Mycol Res 96: 571–577

    Google Scholar 

  • Garbelotto M, Bruns TD, Cobb FW (1993) Differentiation of intersterility groups and geographic provenances among isolates of Heterobasidion annosum detected by randon amplified polymorphic DNA assays. Can J Bot 71: 565–569

    Google Scholar 

  • Goodwin PH, Annis SL (1991) Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assays. Appl Environ Microbiol 57: 2482–2486

    Google Scholar 

  • Jones MJ, Dunkle LD (1993) Analysis of Cochliobolus carbonum races by PCR amplification with arbitrary and gene-specific primers. Mol Plant Pathol 83: 366–370

    Google Scholar 

  • Klein-Lankhorst RM, Vermut A, Weide R, Liharska T, Zabel P (1991) Isolation of molecular markers for tomato (L. esculentum) using random amplified polymorphic DNA (RAPD). Theor Appl Genet 83: 108–114

    Google Scholar 

  • Klittich CJR, Leslie JF (1988) Nitrate reduction mutants of Fusarium moniliforme (Gibberella fujikuroi). Genetics 118: 417–423

    Google Scholar 

  • Klittich CJR, Leslie JF (1989) Chlorate-resistant, nitrate-utilizing (crn) mutants of Fusarium moniliforme (Gibberella fujikuroi). J Gen Microbiol 135: 721–727

    Google Scholar 

  • Kuhlman EG (1982) Varieties of Gibberella fujikuroi with anamorphs in Fusarium section Liseola. Mycologia 74: 759–768

    Google Scholar 

  • Kuhlman EG, Dianis SD, Smith DK (1982) Epidemiology of pitch canker disease in a loblolly-pine seed orchard in North Carolina. Phytopathology 80: 1160–1166

    Google Scholar 

  • Leslie JF (1991) Mating populations in Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 81: 1058–1060

    Google Scholar 

  • Leslie JF, Pearson CAS, Nelson PE, Toussoun TA (1990) Fusarium spp. from corn, sorghum, soybean fields in the central and eastern United States. Phytopathology 80: 343–350

    Google Scholar 

  • Leslie JF, Plattner RD, Desjardins AE, Klittich CJR (1992) Fumonisin B1 production by strains from different mating populations of Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 82: 341–345

    Google Scholar 

  • Matheussen AM, Morgan PW, Frederiksen RA (1990) Implication of gibberellins in head smut (Sporium reilianum) of Sorghum bicolor. Plant Physiol 96: 537–544

    Google Scholar 

  • Megnegneau B, Debets F, Hoekstra RF (1993) Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA. Curr Genet 23: 323–329

    Google Scholar 

  • Meijer G, Megnegneau B, Linders EGA (1994) Variability for isozyme, vegetative compatibility and RAPD markers in natural populations of Phomopsis subordinaria. Mycol Res 98: 267–276

    Google Scholar 

  • Meyer W, Lieckfeldt E, Wöstemeyer J, Börner T (1992) DNA fingerprinting for differentiating aggressivity groups of the rape seed pathogen Leptoshaeria maculans. Mycol Res 96: 651–657

    Google Scholar 

  • Migheli Q, Berio T, Gullino ML (1993) Electrophoretic karyotypes of Fusarium spp. Exp Mycol 17: 329–337

    Google Scholar 

  • Nelson PE (1992) Taxonomy and biology of Fusarium moniliforme. Mycopathologia 117: 29–36

    Google Scholar 

  • Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium species: an illustrated manual for identification. The Pennsylvania State University Press. University Park

    Google Scholar 

  • Nelson PE, Burgess LW, Summerell BA (1990) Some morphological and physiological characters of Fusarium species in sections Liseola and Elegans and similar species. Mycologia 82: 99–106

    Google Scholar 

  • Quellet T, Seifert KA (1993) Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification. Genetics 83: 1003–1007

    Google Scholar 

  • Pegg GF (1984) The role of growth regulators in plant disease. In: Wood RKS, Jellis GJ (eds) Plant diseases: infection, damage and loss. Blackwell Scientific Publications, Oxford, pp 29–48

    Google Scholar 

  • Pontecorvo G, Roper JA, Hemmons LM, MacDonald KD, Bufton AWJ (1953) The genetics of Aspergillus nidulans. Adv Genet 5: 141–238

    Google Scholar 

  • Rollo F, Salvi R, Torchia P (1990) Highly sensitive and fast detection of Phoma tracheiphila by polymerase chain reaction. Appl Microbiol Biotechnol 32: 572–576

    Google Scholar 

  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of β-globin genomic sequences and restriction-site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354

    Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239: 487–491

    Google Scholar 

  • Sambrook J, Pritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York

    Google Scholar 

  • Sarfatti M, Abu-Abied M, Katan J, Zamir D (1991) RFLP mapping of I1, a new locus in tomato conferring resistance against Fusarium oxysporum f.sp. lycopersici race 1. Theor Appl Genet 82: 22–26

    Google Scholar 

  • Schäfer C, Wöstemeyer J (1992) Random-primer dependent PCR differentiates aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Leptosphaeria maculans). J Phytopathol 136: 124–136

    Google Scholar 

  • Schneider G (1988) HPLC von Gibberellinen. In: Sembdner G, Schneider G, Schreiber K (eds) Methoden zur Pflanzenhormonalalyse. Fischer-Verlag, Jena, pp 102–108

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  • Stenlid J, Karlsson JO, Högberg N (1994) Intraspecific genetic variation in Heterobasidion annosum revealed by amplification of minisatellite DNA. Mycol Res 98: 57–63

    Google Scholar 

  • Swofford DL, Maddison WP (1987) Reconstructing ancestral character states under Wagner parsimony. Math Biosci 87: 199–229

    Google Scholar 

  • Takahashi N, Yamaguchi I, Yamane H (1986) Gibberellins. In: Takahashi N (ed) Chemistry of plant homones. CRC Press, Boca Raton, Florida, pp 57–151

    Google Scholar 

  • Takenaka M, Hayashi K, Ogawa T, Kimura S, Tanaka T (1992) Lowered virulence to rice plants and decreased biosynthesis of gibberellins in mutants of Gibberella fujikuroi selected with pefurazoate. J Pest Sci 17: 213–220

    Google Scholar 

  • Vogelstein B, Gillespie D (1979) Preparative and analytical purification of DNA from agarose. Proc Natl Acad Sci USA 76: 615–619

    Google Scholar 

  • Ward E, Akrofi AY (1994) Identification of fungi in the Geaumannomyces-Phialophora complex by RFLPs of PCR-amplified ribosomal DNAs. Mycol Res 98: 219–224

    Google Scholar 

  • Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18: 7213–7218

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535

    Google Scholar 

  • Whitehead DS, Coddington A, Lewis BG (1992) Classification of races by DNA polymorphism analysis and vegetative compatibility grouping in Fusarium oxysporum f.sp. pisi. Physiol Mol Plant Pathol 41: 295–305

    Google Scholar 

  • Wöstemeyer J, Schäfer C, Kellner M, Weisfeld M (1992) DNA polymorphisms detected by random primer-dependent PCR as a powerful tool for molecular diagnostics of plant pathogenic fungi. Adv Mol Gen 5: 227–240

    Google Scholar 

  • Wyss P, Bonfante P (1993) Amplification of genomic DNA of arbuscular-mycorrhizal (AM) fungi by PCR using short arbitrary primers. Mycol Res 97: 1351–1357

    Google Scholar 

  • Yabuta T, Kambe K, Hayashi T (1934) Biochemistry of “bakanae” fungus of rice. J. Agric Chem Soc Japan 10: 1059–1068

    Google Scholar 

Download references

Author information

Author notes

  1. B. Brückner

    Present address: Lehrstuhl für Allgemeine Botanik und Mikrobiologie, Westfälische Wilhelms-Universität, Schlossgarten 3, D-48149, Münster, Germany

Authors and Affiliations

  1. Lebrstuhl für Allgemeine Mikrobiologie und Mikrobengenetik, Fredrich-Schiller-Universität, Neugasse 24, D-07743, Jena, Germany

    K. Voigt, S. Schleier & B. Brückner

Authors
  1. K. Voigt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Schleier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B. Brückner
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Voigt, K., Schleier, S. & Brückner, B. Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD). Curr Genet 27, 528–535 (1995). https://doi.org/10.1007/BF00314443

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00314443

Key words

Search

Navigation