Summary
The role of a notochord fragment on the origin of an additional floor plate area in the neural tube is investigated by quantitative morphological methods. In 1.5 to 2 day chick embryos a notochordal fragment was implanted in close apposition to the lateral wall of the neural groove in the region between prospective wing and leg bud. At 4 days, adjacent to the implant a distinct area of the neural wall was present, which resembled the natural floor plate with respect to its thickness, the abluminal location of elongated nuclei and the absence of neuroblasts. The mitotic density of this area was reduced. This “additional floor plate” was distinct when the experiment was performed at 1.5 days but was hardly recognizable when it was carried out at 2 days.
From these results it is concluded that a) the notochord induces floor plate like structures and diminishes proliferation, and b) that the period of floor plate induction by the notochord is very restricted.
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References
Bergquist H (1959) Experiments on the overgrowth phenomenon in the brain of chick embryos. Anat Anz 106:429–441
Burda DJ (1968) Studies on the experimental induction of overgrowth in chick embryos. Anat Rec 161:419–425
Corliss CE, Robertson GG (1963). The pattern of mitotic density in the early chick neural epithelium. J Exp Zool 153:125–140
Fujita S (1962) Kinetics of cell proliferation. Exp Cell Res 28:52–60
Hamburger V (1948) Kinetics of cell proliferation. Exp Cell Res 28:52–60
Hamburger V (1948) The mitotic pattern in the spinal cord of the chick embryo in relation to histogenetic processes. J Comp Neurol 88:221–284
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Holtfreter J, Hamburger V (1955) Amphibians. In: Willier BH, Weiss PA, Hamburger V (eds) Analysis of development. Saunders, Philadelphia, pp 230–296
Jacob HJ, Christ B, Jacob M, Ahlström P (1976) Differenzierung des Neuralrohres. Experimentelle Untersuchungen an Hühnerund Wachtelembryonen. Acta Anat 94:204–220
Jacobson AG (1978) Some forces that shape the nervous system. Zoon 6:13–21
Jacobson AG, Oster GF, Odell GM, Cheng LY (1986) Neurulation and the cortical tractor model for epithelial folding. J Embryol Exp Morphol 96:19–49
Jacobson C-O (1962) Cell migration in the neural plate and the process of neurulation in the axolotl larva. Zool Bidrag Uppsala 35:433–449
Jacobson M (1979) Developmental Neurobiology (2nd edn) Plenum Press, New York
Källén B (1965) Early Morphogenesis and pattern formation in the central nervous system. In: de Haan R, Ursprung H (eds) Organogenesis. Holt, Rhinehart and Winston, New York, pp 107–128
Karfunkel P (1974) The mechanism of neural tube formation. Int Rev Cytol 38:245–271
Karnovski MJ, Roots L (1964) A “direct colouring” thiocholine method for cholinesterase. J Histochem Cytochem 12:219–221
Limborgh J van (1956) The influence of adjacent mesodermal structures upon the shape of the neural tube and plate in bird embryos. Acta Morphol Neerl Scand 1:155–166
Martin AH (1977) Neural tube — somite interaction, effect of neural tube reversal upon neuron development. Acta Embryol Exp 3:305–313
Miki A, Atoji Y, Mizoguti H (1981) The relationship between the proliferating ability and acetylcholinesterase activity of the neural tube cells in early chick embryos. Acta Histochem Cytochem 14:461–475
Mitolo V (1967) Quantitative data on the mitotic activity in the brachial and thoracic segments of the chick embryo neural tube from the 4th to the 8th day. Acta Embryol Morphol Exp 10:62–74
Sauer FC (1935) Mitosis in the neural tube. J Comp Neurol 62:377–405
Schoenwolf GC, Franks MV (1984) Quantitative analysis of changes in cell shapes during bending of the avian neural plate. Dev Biol 105:257–272
Schoenwolf GC (1985) Shaping and bending of the avian neuroepithelium: morphometric analyses. Dev Biol 109:127–139
Steding G (1962) Experimente zur Morphogenese des Rückenmarkes. Acta Anat 49:199–231
Straaten HWM van, Thors F, Hoessels EL, Hekking JWM, Drukker J (1985a) Effect of a notochordal implant on the early morphogenesis of the neural tube and neuroblasts. Histometrical and histological results. Dev Biol 110:247–254
Straaten HWM van, Hekking JWM, Thors F, Wiertz ELJM, Drukker J (1985b) Induction of an additional floor plate in the neural tube. Acta Morphol Neerl Scand 23:91–97
Straaten HWM van, Hekking JWM, Drukker J (1986) The demonstration of acetylcholinesterase in plastic sections. Its application as a marker of early neuronal development. Acta Histochem Suppl 32:185–190
Straaten HWM, van Drukker J (1987a) Influence of the notochord on the morphogenesis of the neural tube. In: Wolff JR (ed) Mesenchymal-epithelial interactions. Academic Press, pp 153–163
Straaten HWM van, Hekking J, Drukker J (1987b) A toluidin blue staining procedure selective for nuclei, mitotic figures and axial processes in GMA embedded embryonic neural tissue. Stain Technol (in press)
Thors F, Kort EMJ de, Nieuwenhuys R (1982) On the development of the spinal cord of the clawed frog, Xenopus laevis. I. Morphogenesis and histogenesis. Anat Embryol 164:427–441
Watterson RL, Goodheart C, Lindberg G (1955) The influence of adjacent structures upon the shape of the neural tube and neural plate of chick embryos. Anat Rec 122:539–559
Watterson RL (1965) Structure and mitotic behavior of the early neural tube. In: Haan RL de, Ursprung H (eds) Organogenesis. Holt, Rhinehart and Winston, New York Chicago, pp 129–159
Wiertz-Hoessels ELMJ, Hara K, Hekking JWM, van Straaten HWM, Thors F, Drukker J (1987) Differentiation of gut entoderm in dependence of the notochord. Anat Embryol 176:337–343
Youn BW, Malacinsky GM (1981) Axial structure development in ultraviolet-irradiated (notochord-defective) amphibian embryos. Dev Biol 83:339–352
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van Straaten, H.W.M., Hekking, J.W.M., Wiertz-Hoessels, E.J.L.M. et al. Effect of the notochord on the differentiation of a floor plate area in the neural tube of the chick embryo. Anat Embryol 177, 317–324 (1988). https://doi.org/10.1007/BF00315839
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DOI: https://doi.org/10.1007/BF00315839