Summary
The intestine of Aldrichetta forsteri was examined ultrastructurally for evidence of 5-HT-containing intrinsic neurons. A population of nerve cell bodies and processes characterized by the presence of amine-containing vesicles was found after the destruction of adrenergic processes with 6-hydroxydopamine. The distribution of the neurons matched that of the 5-HT-containing neurons previously seen with formaldehyde-induced fluorescence and 5-HT immunohistochemistry in A. forsteri and other teleosts. The axons made close contacts with the circular smooth muscle layer and the muscularis mucosae. The axons also lay exposed to the epithelial cells of the mucosa but did not make contact with neurons or with the longitudinal smooth muscle layer.
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References
Anderson CR (1983) Evidence for 5-HT-containing neurons in the teleost intestine. Cell Tissue Res 230:377–386
Anderson CR, Campbell GD (1988) Immunohistochemical study of 5-HT-containing neurones in the teleost intestine: relationship to presence of enterochromaffin cells. Cell Tissue Res 254:553–559
Baumgarten HG, Björklund A, Lachenmeyer L, Nobin A, Rosengren E (1973) Evidence for the existence of serotonin-, dopamine-, and noradrenaline-containing neurons in the gut of Lampetra fluviatilis. Z Zellforsch Mikrosk-Anat 141:33–54
Burnstock G, Costa M (1975) Adrenergic neurons. Chapman and Hall, London
Cuello AC (1982) Co-transmission. Macmillan Press, London
Ezeasor DN (1979) Ultrastructural observations on the submucous plexus of the large intestine of the rainbow trout (Salmo gairdneri, Rich). Z Mikrosk Anat Forsch 93:803–812
Fange R, Grove D (1979) Digestion. In: Randall DJ, Fange R (eds) Fish Physiology, Vol VIII. Academic, New York, pp 162–260
Fried G (1982) Neuropeptide storage in vesicles. In: Klein RK, Lagerkrantz H, Zimmerman H (eds) Neurotransmitter vesicles. Academic Press, London, pp 361–374
Fried G, Terenius L, Brodin E, Efendic S, Dockray G, Fahrenkrug J, Goldstein M, Hökfelt T (1986) Neuropeptide Y, enkephalin and noradrenaline coexist in sympathetic neurons innervating the bovine spleen. Biochemical and immunohistochemical evidence. Cell Tissue Res 243:495–508
Furness JB, Costa M (1982) Neurons with 5-hydroxytryptaminelike immunoreactivity in the enteric nervous system: their projections in the guinea-pig small intestine. Neuroscience 7:341–349
Gabella G (1972) Innervation of the intestinal muscle coat. J Neurocytol 1:341–362
Gershon MD, Sherman DL (1982) Identification of the interactions between noradrenergic and serotonergic neurites in the myenteric plexus. J Comp Neurol 204:402–421
Gibbins IL (1982) Lack of correlation between ultrastructural and pharmacological types of non-adrenergic autonomic nerves. Cell Tissue Res 221:551–581
Gibbins IL, Haller CJ (1979) Ultrastructural identification of nonadrenergic, non-cholinergic nerves in the rat anococcygeus muscle. Cell Tissue Res 200:257–271
Gordon-Weeks PR (1982) Noradrenergic and non-noradrenergic nerves containing small granular vesicles in Auerbach's plexus of the guinea pig: evidence against the presence of noradrenergic synapses. Neuroscience 7:2925–2936
Hopwood D (1971) The histochemistry and electron histochemistry of chromaffin tissue. Prog Histochem Cytochem 3:1–66
Jonakait GM, Tamir H, Gintzler AR, Gershon MD (1979) Release of (3H) serotonin and its binding protein from enteric neurons. Brain Res 174:55–69
Julé Y (1980) Nerve mediated descending inhibition in the proximal colon of the rabbit. J Physiol 309:487–498
Klein RL, Lagercrantz H, Zimmerman H (1982) Neurotransmitter vesicles. Academic Press, New York
Lagercrantz H (1976) On the composition and function of large dense cored vesicles in sympathetic nerves. Neuroscience 1:81–92
Lagercrantz H, Thureson-Klein Å (1975) On the soluble phase of adrenergic nerve vesicles: correlation of matrix density and biochemical composition. Histochemistry 43:173–183
Llewellyn-Smith IJ, Wilson AJ, Furness JB, Costa M, Rush RA (1981) Ultrastructural identification of noradrenergic axons and their distribution within the enteric plexuses of the guineapig small intestine. J Neurocytol 10:331–352
Manber L, Gershon MD (1979) A reciprocal adrenergic-cholinergic axoaxonic synapse in the mammalian gut. Am J Physiol 236:E738-E745
Pelletier G, Steinbusch HW, Verhofstad AA (1981) Immunoreactive substance P and serotonin present in the same dense-core vescile. Nature 293:71–72
Richards JG (1982) Ultrastructural visualization of biogenic amines. In: Hökfelt T, Björklund A (eds) Handbook of chemical neuroanatomy, Vol I. Elsevier, Amsterdam, pp 122–146
Tamir H, Huang IL (1974) Binding of serotonin to soluble protein from synaptosomes. Life Sci 14:83–93
Tamir H, Kuhar MJ (1975) Association of serotonin-binding protein with projection of the midbrain raphe nuclei. Brain Res 83:164–172
Thureson-Klein Å (1983) Exocytosis from large and small dense cored vesicles in noradrenergic nerve terminals. Neuroscience 10:245–260
Tranzer JP, Richards JG (1976) Ultrastructural cytochemistry of biogenic amines in nervous tissue: methodological improvements. J Histochem Cytochem 24:1178–1193
Tranzer JP, Thoenen H (1967) Electronmicroscopic localization of 5-hydroxydopamine (3,4,5 trihydroxy-phenyl-ethylamine), a new false transmitter. Experientia 23:743–745
Tranzer JP, Thoenen H (1968) Various types of amine storage vesicles in peripheral adrenergic nerve terminals. Experientia 24:484–486
Watson AHD (1979) Fluorescent histochemistry of the teleost gut: evidence for the presence of serotonergic neurons. Cell Tissue Res 197:155–164
Watson AHD (1981) The ultrastructure of the innervation of the intestinal wall in the telcosts Myoxocephalus scorpius and Pleuronectes platessa. Cell Tissue Res 214:651–658
Wong WC, Tan CK (1978) Fine structure of the myenteric and submucous plexuses in the stomach of a coral fish, Chelmon rostratus, Cuvier. J Anat 124:637–642
Wood RI (1977) Changes in sizes of the adrenaline-containing vesicles and their cores in cardiac sympathetic nerves after pharmacological treatments. J Neurocytol 6:375–396
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Anderson, C.R. The ultrastructure and distribution of 5-HT-containing neurons in the intestine of a teleost fish, Aldrichetta forsteri . Cell Tissue Res 259, 379–387 (1990). https://doi.org/10.1007/BF00318461
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DOI: https://doi.org/10.1007/BF00318461