Abstract
The location of satellite DNA sequences in metaphase chromosomes has been studied in the kangaroo rat by the in situ hybridization technique, staining techniques and phase contrast microscopy. The HS-β satellite DNA is located at the kinetochores of all but three chromosome pairs. The HD satellite is located predominantly in the short arms of the chromosomes containing HS-β and in the kinetochores of chromosome pairs that lack HS-β. The regions that contain the satellite DNA sequences can also be identified by the Giemsa staining technique, and can be visualized with phase contrast microscopy or following Feulgen staining of fixed chromosome preparations.
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Arrighi, F.E., Hsu, T.C.: Localization of heterochromatin in human chromosomes. Cytogenetics 10, 81–86 (1971).
Bostock, C.J., Prescott, D.M., Hatch, F.T.: The timing of replication of the satellite and main band DNAs in cells of the kangaroo rat (Dipodomys ordii). Exp. Cell Res. 74, 487–495 (1972).
Gall, J.G., Pardue, M.L.: Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc. nat. Acad. Sci. (Wash.) 63, 378–383 (1969).
Gall, J.G., Pardue, M.L.: Nucleic acid hybridization in cytological preparations. Meth. Enzymol. 21D, 470–480 (1971).
Hatch, F.T., Mazrimas, J.A.: Satellite DNAs in the kangaroo rat. Biochim. biophys. Acta (Amst.) 224, 291–294 (1970).
Kay, E.R.M., Simmons, N.S., Dounce, A.L.: An improved preparation of sodium desoxyribonucleate. J. Amer. chem. Soc. 74, 1724–1726 (1952).
Mazrimas, J.A., Hatch, F.T.: Intranuclear distribution of satellite DNA from kangaroo rat. Exp. Cell Res 63, 462–466 (1970).
Mazrimas, J.A., Hatch, F.T.: Separation of multiple nuclear satellite DNAs in the kangaroo rat. Fed. Proc. 30, 1177 Abstr. (1971).
Pardue, M.L., Gall, J.G.: Chromosomal localization of mouse satellite DNA. Science 168, 1356–1358 (1970).
Pardue, M.L., Gerbi, S.A., Eckhardt, R.A., Gall, J. G.: Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma (Berl.) 29, 268–290 (1970).
Salomon, R., Kaye, A.M., Herzberg, M.: Mouse nuclear satellite DNA: 5-methylcytosine content, pyrimidine isoplith distribution and electron microscopic appearance. J. molec. Biol. 43, 581–592 (1969).
Saunders, G.F., Shirakawa, S., Saunders, P.P., Arrighi, F.E., Hsu, T.C.: Populations of repeated DNA sequences in the human genome. J. molec. Biol. 63, 323–334 (1972).
Swift, H.: Cytochemical techniques for nucleic acids. The nucleic acids (E. Chargaff and J.N. Davidson, eds.), vol. 2, p. 51–92. New York: Academic Press 1955.
Walker, P.M.B.: “Repetitive” DNA in higher organisms. Prog. Biophys. and molec. Biol. 23, 145–190 (1971).
Yunis, J.J., Roldan, L., Yasmineh, W.G., Lee, J.G.: Staining of satellite DNA in metaphase chromosomes. Nature (Lond.) 231, 532–533 (1971).
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Prescott, D.M., Bostock, C.J., Hatch, F.T. et al. Location of satellite DNAs in the chromosomes of the kangaroo rat (Dipodomys ordii). Chromosoma 42, 205–213 (1973). https://doi.org/10.1007/BF00320941
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DOI: https://doi.org/10.1007/BF00320941