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Localization of neuropeptide Y and atrial natriuretic peptide in the endothelial cells of human umibilical blood vessels

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Abstract

The localization of neuropeptide Y (NPY) and atrial natriuretic peptide (ANP) in the endothelial cells of human umbilical blood vessels was studied using the pre-embedding peroxidase-antiperoxidase (PAP) technique for electron microscopy and avidin-biotin-complex (ABC) immunostaining for endothelial cells cultured from umbilical vein. Subpopulations of NPY- and ANP-immunoreactive endothelial cells were present in term umbilical vein and artery. The umbilical vein contained more positive cells than the artery. The percentage of NPY- and ANP-immunoreactive umbilical vein cells in culture was 32% and 44%, respectively, out of a total of 3013 cells examined. The possibility that these potent vasoactive substances located in the endothelial cells of the non-innervated umbilical vessels are involved in the local regulation of blood flow is discussed.

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Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London, United Kingdom

    W. Q. Cai, P. Bodin, A. Sexton, A. Loesch & G. Burnstock

  2. Centre for Neuroscience, University College London, Gower Street, WC1E 6BT, London, United Kingdom

    W. Q. Cai, P. Bodin, A. Sexton, A. Loesch & G. Burnstock

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  1. W. Q. Cai
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  2. P. Bodin
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  3. A. Sexton
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  4. A. Loesch
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  5. G. Burnstock
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Cai, W.Q., Bodin, P., Sexton, A. et al. Localization of neuropeptide Y and atrial natriuretic peptide in the endothelial cells of human umibilical blood vessels. Cell Tissue Res 272, 175–181 (1993). https://doi.org/10.1007/BF00323584

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  • DOI: https://doi.org/10.1007/BF00323584

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