Summary
Just before ovulation we treated 10–12-week-old female mice of the strains C3H and (101×C3H)F1 with 2.3.5.-triethyleneimonobenzoquinone-1.4 (T), cyclophosphamide (C) and methotrexat (M). The chromosome analysis took place shortly after ovulation at the time of metaphase II. The method described in detail seems to be appropriate to examine the induction of genetic defects during oogenesis by chemical agents.
Zusammenfassung
Wir behandelten 10–12 Wochen alte weibliche Mäuse der Stämme C3H und (101×C3H)F1 kurz vor der Ovulation mit 2.3.5.-Triethyleniminobenzochinon-1.4 (T), Cyclophosphamid (C) und Methotrexat (M) Die Chromosomenanalyse erfolgte kurz nach der Ovulation zum Zeitpunkt der Metaphase II. Die im Detail beschriebene Methode erscheint geeignet, die Auslösung genetisch bedingter Eireifungsstörugen durch chemische Agentien zu untersuchen.
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Röhrborn, G., Hansmann, I. Induced chromosome aberrations in unfertilized oocytes of mice. Humangenetik 13, 184–198 (1971). https://doi.org/10.1007/BF00326941
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DOI: https://doi.org/10.1007/BF00326941