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Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli

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Summary

A mutation (lspA, prolipoprotein signal peptidase) rendering the prolipoprotein signal peptidase temperature-sensitive in Escherichia coli has been analyzed. The mutation was mapped in the dnaJ-rpsT-ileS-dapB region by interrupted mating with various Hfr strains and P1 phage transduction. λ transducing phage λddapB2 that carries the rpsT-ileS-dapB region was shown to complement the lspA mutation. Plasmid pLC3-13 which had been isolated from Clarke and Carbon's collection as a plasmid carrying the lspA locus was shown to carry the dnaJ and rpsT loci. Complementation analysis with plasmids carrying various DNA fragments derived from pLC3-13 showed that the lspA locus is between the rpsT and ileS loci. The wildtype allele was dominant over the lspA allele.

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Authors and Affiliations

  1. Laboratory of Fermentation Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, 464, Nagoya, Japan

    Hideo Yamagata & Nana Taguchi

  2. Laboratory of Microbiology, Faculty of Agriculture, Nagoya University, Chikusa-ku, 464, Nagoya, Japan

    Kyoko Daishima & Shoji Mizushima

Authors
  1. Hideo Yamagata
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  2. Nana Taguchi
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  3. Kyoko Daishima
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  4. Shoji Mizushima
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Communicated by K. Isono

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Yamagata, H., Taguchi, N., Daishima, K. et al. Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli . Molec Gen Genet 192, 10–14 (1983). https://doi.org/10.1007/BF00327640

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  • DOI: https://doi.org/10.1007/BF00327640

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