Summary
The nucleotide sequence of the putP gene coding for the proline carrier in Escherichia coli has been determined and the amino acid sequence of the proline carrier deduced from it. The proline carrier is predicted to consist of 502 amino acids, resulting in a molecular weight of 54,343. The predicted protein is very hydrophobic (70% nonpolar amino acids), and its hydropathy profile suggests that it is composed of 12 hydrophobic segments with a mean length of 24.4 residues/segment. If these segments are assumed to be α-helical, the mean length of each domain corresponds to the thickness of the hydrophobic core of the membrane. Potential promoter, catabolite gene activator protein (CAP) binding sites and several palindromic sequence, which might be regulatory regions by the putA gene product, were also found in the 5′ flanking region of the postulated putP gene. A typical p-independent transcription termination signal was found after the terminator codon of the putP gene.
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References
Büchel DE, Gronenborn B, Müller-Hill B (1980) Sequence of the lactose permease gene. Nature 283:541–545
Capaldi RA, Vanderkooi G (1972) The low polarity of many membrane proteins. Proc Natl Acad Sci USA 69:930–932
Casadaban MJ, Chou J, Cohen SN (1980) In vitro gene fusions that join an enzymatically active β-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol 143:971–980
Chou PY, Fasman GD (1978) Prediction of the secondary structure of proteins from their amino acid sequence. Adv Enzymol 47:45–148
Condamine H (1971) Mutans des voies de biosynthése et de dégradation de la proline chez E. coli K12. Ann Inst Pasteur (Paris) 120:9–22
Finer-Moore J, Stroud RM (1984) Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor. Proc Natl Acad Sci USA 81:155–159
Foster DL, Boublik M, Kaback HR (1983) Structure of the lac carrier protein of Escherichia coli. J Biol Chem 258:31–34
Graham SB, Stephanson JT, Wood JM (1984) Proline dehydrogenase from Escherichia coli K12. Reconstitution of a functional membrane association. J Biol Chem 259:2656–2661
Hanada K, Yamato I, Anraku Y (1985) Identification of proline carrier in Escherichia coli K12. FEBS Lett 191:278–282
Ikemura T (1981) Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system. J Mol Biol 151:389–409
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Messing J (1983) New M13 vectors for cloning. Methods Enzymol 101:20–78
Mogi T, Anraku Y (1984a) Mechanism of proline transport in Escherichia coli K12. I. Effect of a membrane potential on the kinetics of 2H+/proline symport in cytoplasmic membrane vesicles. J Biol Chem 259:7791–7796
Mogi T, Anraku Y (1984b) Mechanism of proline transport in Escherichia coli K12. II. Effect of alkaline cations on binding of proline to a H+/proline symport carrier in cytoplasmic membrane vesicles. J Biol Chem 259:7796–7801
Mogi T, Anraku Y (1984c) Mechanism of proline transport in Escherichia coli K12. III. Inhibition of membrane-potentialdriven proline transport by syn-coupled ions and evidence for symmetrical transition states of the 2H+/proline symport carrier. J Biol Chem 259:7801–7806
Mogi T, Yamamoto H, Nakao T, Yamato I, Anraku Y (1986) Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12. Mol Gen Genet 202:35–41
Motojima K, Yamato I, Anraku Y (1978) Proline transport carrierdefective mutants of Escherichia coli K12: Properties and mapping. J Bacteriol 136:5–9
O'Neill MC, Amass K, de Crombrugghe B (1981) Molecular model of the DNA interaction site for the cyclic AMP receptor protein. Proc Natl Acad Sci USA 78:2213–2217
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Wood JM (1981) Genetics of L-proline utilization in Escherichia coli. J Bacteriol 146:895–901
Wood JM, Zadworny D (1979) Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12. Can J Biochem 57:1191–1199
Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
Yazyu H, Shiota-Niiya S, Shimamoto T, Kanazawa H, Futai M, Tsuchiya T (1984) Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem 259:4320–4326
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Nakao, T., Yamato, I. & Anraku, Y. Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12. Mole Gen Genet 208, 70–75 (1987). https://doi.org/10.1007/BF00330424
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DOI: https://doi.org/10.1007/BF00330424