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Degeneration and regeneration of smooth muscle transplants in the anterior eye chamber

An ultrastructural study

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Summary

Smooth muscle from small segments of the vas deferens, urinary bladder and the ureter of the guinea pig was transplanted into the anterior chamber of the eye. Some muscle cells had a normal appearance, but others showed morphological changes during the first few days following transplantation. Changes of two main kinds were observed: some cells contained aggregations of glycogen granules and lipid droplets; other cells were characterized by either a general increase in electron density of the cell with the presence of very thick filaments (which are, possibly, aggregates of thin filaments), or cells containing an electron-dense nucleus, ruptured cellular membranous systems and degenerating mitochondria. Following revascularisation, which commenced one to two days after transplantation, areas containing cells with an appearance similar to that of embryonic smooth muscle were apparent. Evidence of mitosis of smooth-muscle and other cell types was also noted, the most active period being five to ten days after transplantation. Bundles containing normal muscle cells were apparent by four weeks in these transplants. However, whole undissected transplants contained normal-looking muscle bundles after one week. These findings are discussed in relation to the repair of wounds to smooth muscle.

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Authors and Affiliations

  1. Department of Zoology, University of Melbourne, 3052, Parkville, Victoria, Australia

    Gordon R. Campbell, Yasuo Uehara, Torbjörn Malmfors & Geoffrey Burnstock

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  1. Gordon R. Campbell
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  2. Yasuo Uehara
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  3. Torbjörn Malmfors
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  4. Geoffrey Burnstock
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Additional information

This work was supported by the National Heart Foundation of Australia; the Australian Research Grants Committee and the Swedish Medical Research Council Grant B69-14X-711-05, 14 R-2697.

T. Malmfors was the Warren MacDonald International Fellow of the National Heart Foundation of Australia at the time of this study.

We are grateful to Dr. N. C. R. Merrillees for his constructive criticisms of the manuscript, and we are indebted to Miss Ulla Enberg, Miss Janet McConnell and to Mrs. Vicki James for their excellent technical assistance.

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Campbell, G.R., Uehara, Y., Malmfors, T. et al. Degeneration and regeneration of smooth muscle transplants in the anterior eye chamber. Z. Zellforsch. 117, 155–175 (1971). https://doi.org/10.1007/BF00330735

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  • DOI: https://doi.org/10.1007/BF00330735

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