Summary
A simple method for the preparation of ciliated epithelia for study with the scanning electron microscope is described. Ciliary groups are well preserved and it is possible to discern individual cilia and work out their numbers and orientation. Following scanning electron microscopical study some of the material was prepared for transmission electron microscopy and the ultrastructure of the tissue was found to be surprisingly well preserved. The tracheal epithelium of the rabbit, the olfactory epithelia of the goldfish and the rabbit, and the sensory epithelia in the statocyst of a cephalopod mollusc were examined with the scanning electron microscope to demonstrate the possibilities of the method.
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References
Allison, A. C.: The morphology of the olfactory system in vertebrates. Biol. Eev. 28, 195–244 (1953).
—, and R. T. Turner Warwick: Quantitative observations on olfactory system of rabbit. Brain 72, 186–197 (1949).
Bannister, L. H.: The fine structure of the olfactory surface of teleostean fishes. Quart. J. micr. Sci. 106, 333–342 (1965).
Barber, V. C.: Preliminary observations on the fine structure of the Octopus statocyst. J. Microsc. 4, 547–550 (1965).
—: The fine structure of the statocyst of Octopus vulgaris. Z. Zellforsch. 70, 91–107 (1966a).
—: The morphological polarisation of kinocilia in the Octopus statocyst. J. Anat. Lond., 100, 685–686 (1966b).
- The structure of mollusc statocysts, with particular reference to cephalopods. Symp. Zool. Soc., Lond. In Press (1968).
Blümcke, S., and K. Morgenroth jr.: The stereo ultrastructure of the external and internal surface of the cornea. J. Ultrastruct. Res. 18, 502–518 (1967).
Boyde, A.: Direct visualisation of the site of development of enamel prism sheaths. Naturwissenschaften 54, 252 (1967a).
—: A single-stage carbon-replica method and some related techniques for the analysis of the electron microscope image. J. roy. micr. Soc. 86, 359–370 (1967b).
Boyde, A., and K. S. Lester: An electron microscope study of fractured dentural surfaces. Calc. Tiss. Res. 1, 122–136 (1967a).
—: The structure and development of marsupial enamel tubules. Z. Zellforsch. 82, 558–576 (1967b).
—: Electron microscopy of resorbing surfaces of dental hard tissues. Z. Zellforsch. 83, 538–548 (1967c).
—, and A. D. G. Stewart: Scanning electron microscopy of the surface of developing mammalian dental enamel. Nature (Lond.). 198, 1102–1103 (1963).
Clarke, J. A., and A. J. Salsbury: Surface ultramicrosoopy of human blood cells. Nature (Lond.) 215, 402–404 (1967).
Fawcett, D. W., and K. R. Porter: A study of the fine structure of ciliated epithelia. J. Morph. 94, 221–282 (1954).
Flock, Å.: Transducing mechanisms in the lateral line canal organ receptor. Cold Spr. Harb. Symp. quant. Biol. 30, 133–145 (1965).
Glauert, A. M., and R. H. Glauert: Araldite as an embedding medium for electron microscopy. J. biophys. biochem. Cytol. 4, 191–194 (1958).
Graziadei, P.: Electron microscopic observations of the olfactory mucosa of the mole. J. Zool. (Lond.) 149, 89–94 (1966).
Holl, A.: Vergleichende morphologische und histologische Untersuchungen am Geruchsorgan der Knochenfische. Z. Morphol. Ökol. Tiere, 54, 707–782 (1965).
Laverack, M. S.: On superficial receptors. Symp. Zool. Soc. Lond. (In Press) (1968).
Le Gros Clark, W. E.: Observations on the structure and organization of olfactory receptors in the rabbit. Yale J. Biol. Med. 29, 83–95 (1956).
Lester, K. S., and A. Boyde: Electron microscopy of predentinal surfaces. Calc. Tiss. Res. 1, 44–54 (1967).
Lorenzo, A. J. De: Electron microscopic observations of the olfactory mucosa and olfactory nerve. J. biophys. biochem. Cytol. 3, 839–850 (1957).
Luft, J. H.: Permanganate — a new fixative for electron microscopy. J. biophys. biochem. Cytol. 2, 799–801 (1956).
McDonald, L.W., R. F. W. Pease, and T. L. Hayes: Scanning electron microscopy of sectioned tissue. Lab. Invest. 16, 532–538 (1967).
Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Phys. 32, 1637 (1961).
Okano, M., A. F. Weber, and S. P. Frommes: Electron microscopic studies of the distal border of the canine olfactory epithelium. J. Ultrastruct. Res. 17, 487–502 (1967).
Porter, K. R., and M. A. Bonneville: An introduction to the fine structure of cells and tissues. Philadelphia: Lea & Febiger 1964.
Reese, T. S.: Olfactory cilia in the frog. J. Cell Biol. 25, 209–230 (1965).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Stewart, A. D. G., and A. Boyde: Ion etching of dental tissues in a scanning electron microscope. Nature (Lond.) 196, 81–82 (1962).
Thornley, R. F. M.: New applications of the scanning electron microscope. Ph. D. Thesis, University of Cambridge (1960).
Trujillo-Cenóz, O.: Electron microscope observations on chemo- and mechano-receptor cells of fishes. Z. Zellforsch. 54, 654–676 (1961).
Tucker, D.: Olfactory cilia are not required for receptor function. Fed. Proc. 26, 544 Abstract 1609 (1967).
Wersäll, J., Å. Flock, and P.-G. Lundquist: Structural basis for directional sensitivity in cochlear and vestibular sensory receptors. Cold. Spr. Harb. Symp. quant. Biol. 30, 115–132 (1965).
Young, J. Z.: The statocysts of Octopus vulgaris. Proc. roy. Soc. B 152, 3–29 (1960).
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Acknowledgements. We would like to thank Professor J. Z. Young for his continued interest and support. The scanning electron microscope was purchased with a grant provided by the Science Research Council to Dr. Boyde, Mr. R. Willis helped in the initial stages of the study, Mr. G. Savage provided help with the goldfish material, Mr. S. Waterman provided much photographic assistance, and Mrs. N. Finney the secretarial assistance.
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Barber, V.C., Boyde, A. Scanning electron microscopic studies of cilia. Z. Zellforsch. 84, 269–284 (1967). https://doi.org/10.1007/BF00330870
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DOI: https://doi.org/10.1007/BF00330870