Summary
A pBR322 recombinant plasmid containing the spinach chloroplast DNA fragment SalI-5 has been used for coupled transcription-translation. Translational products of 19, 16 and 12x103 daltons give a positive immunoreaction with mixed antibodies against 30S and 50S spinach chloroplast ribosomal proteins. Three translational products co-migrate with basic chloroplast ribosomal proteins separated by two-dimensional electrophoresis. It is concluded that at least three ribosomal proteins are encoded by the SalI-5 DNA fragment, probably CS-S19, CS-L24 and CS-L25.
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Abbreviations
- SDS:
-
sodium dodecyl sulphate
- CS-L and CS-S:
-
spinach chloroplast large and small ribosomal subunits, respectively
- cpDNA:
-
chloroplast DNA
- d:
-
daltons
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Communicated R.G. Herrmann
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Dorne, A.M., Heizmann, P., Alt, J. et al. Spinach chloroplast genes coding for three ribosomal proteins. Mol Gen Genet 198, 521–524 (1985). https://doi.org/10.1007/BF00332950
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DOI: https://doi.org/10.1007/BF00332950