Summary
The synthesis of two enzymes of the arginine catabolic pathway, arginase and ornithine δ-transaminase (OTAse), in Aspergillus nidulans was found to be sensitive to both glucose and ammonium repression. The glucose and nitrogen starvation result in the identical derepression of OTAse synthesis and have no effects on arginase synthesis. Glucose and ammonium affect the kinetics of induction of both enzymes, however, the effect of ammonium is much stronger. Evidence was obtained for the direct involvement of ammonium in the repression phenomenon. The relations between glucose and ammonium repression are discussed.
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References
Arst, H. N., Cove, D. J.: Methylammonium resistance in Aspergillus nidulans. J. Bact. 98, 1284–1293 (1969)
Cove, D. J.: The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 113, 51–56 (1966)
Cybis, J., Piotrowska, M., Weglenski, P.: Control of ornithine transcarbamylase formation in Aspergillus nidulans. Bull. Acad. pol. Sci., Ser. Sci. Biol. 18, 669–672 (1970)
Cybis, J., Piotrowska, M., Weglenski, P.: Genetic control of the arginine pathways in Aspergillus nidulans. Common regulation of anabolism and catabolism. Molec. gen. Genet. 118, 272–277 (1972)
Cybis, J., Weglenski, P.: Arginase induction in Aspergillus nidulans. The appearance and decay of coding capacity of messenger. Europ. J. Biochem. 30, 262–268 (1972)
Eliasson, E. E.: Regulation of arginase activity in Chang's liver cells in tissue culture. Biochim. biophys. Acta (Amst.) 97, 449–459 (1965)
Grenson, M., Hou, C.: Ammonia inhibition of the general amino acid permease and its suppression in NADPH-specific glutamate dehydrogenaseless mutants of Saccharomyces cerevisiae. Biochem. biophys. Res. Commun. 48, 749–755 (1972)
Hynes, M. J.: Induction and repression of the amidase enzymes in Aspergillus nidulans. J. Bact. 103, 482–487 (1970)
Laishley, E. J., Bernlohr, R. W.: Regulation of arginine and proline catabolism in Bacillus licheniformis. J. Bact. 96, 322–329 (1968)
Lowry, O. H., Rosebrough, M. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Magasanik, B.: Nutrition of bacteria and fungi. Ann. Rev. Microbiol. 11, 221–252 (1957)
Magasanik, B.: Glucose effects: inducer exclusion and repression. In: The lactose operon (J. R. Beckwith and D. Zipser eds.), p. 189–220. Cold Spring Harbor: Cold Spring Harbor Laboratory 1970
Middelhoven, W. J.: Induction and repression of arginase and ornithine transaminase in baker's yeast. Antonie van Leeuwenhoek 36, 1–19 (1970)
Pastan, I., Perlman, R.: Cyclic adenosine monophosphate in bacteria. Science 169, 339–344 (1970)
Scazzocchio, C., Darlington, A. J.: The induction and repression of the enzymes of purine breakdown in Aspergillus nidulans. Biochim. biophys. Acta (Amst.) 166, 557–568 (1968)
Weglenski, P.: The mechanism of action of proline suppressors in Aspergillus nidulans. J. gen. Microbiol. 47, 77–85 (1967)
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Communicated by F. Kaudewitz
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Bartnik, E., Weglenski, P. & Piotrowska, M. Ammonium and glucose repression of the arginine catabolic enzymes in Aspergillus nidulans . Molec. gen. Genet. 126, 75–84 (1973). https://doi.org/10.1007/BF00333484
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DOI: https://doi.org/10.1007/BF00333484