Summary
Electron micrographs of trypsin-dissociated rat adrenal showed predominantly intact rounded cells without internal damage. The population contained cells from the glomerular, intermediary and fascicular zones with cells from the zona fasciculata predominant. The presence or absence of cells from the reticular zone could not be determined. Cells from the medullary zone were absent. The addition of adrenocorticotropic hormone (ACTH) to the cellular suspension for 2 hours produced corticosterone. However, these stimulated cells did not display any significant ultrastructural change.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bangham, D. R., Mussett, M. V., Stack-Dunne, M. P.: The third international standard for corticotrophin. Bull. Wld Hlth Org. 27, 395–408 (1962).
Fawcett, D. W., Long, J. A., Jones, A. L.: The ultrastructure of endocrine glands. Recent Progr. Hormone Res. 25, 315–380 (1969).
Halkerston, I. D. K., Feinsten, M., Hechter, O.: Effect of lytic enzymes upon the responsivity of rat adrenals in vitro. I. Effect of trypsin upon the steroidogenic action of reduced triphosphopyridine nucleotide. Endocrinology 83, 61–73 (1968).
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol. 9, 409–414 (1961).
Luse, S.: Fine structure of adrenal cortex. In: The adrenal cortex, 1st edit. (Eisenstein, A. B., ed.), p. 1–59. Boston: Little, Brown & Company 1967.
Malamed, S.: Use of a microcentrifuge for preparation of isolated mitochondria and cell suspensions for electron microscopy. J. Cell Biol. 18, 696–700 (1963).
Palade, G. E.: A study of fixation for electron microscopy. J. exp. Med. 95, 285–298 (1952).
Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963).
Sabatini, D. D., De Robertis, E. D. P.: Ultrastructural zonation of adrenocortex in the rat. J. biophys. biochem. Cytol. 9, 105–119 (1961).
Silber, R. H., Busch, R. D., Oslapas, R.: Practical procedure for estimation of corticosterone or hydrocortisone. Clin. Chem. 4, 278–285 (1958).
Swallow, R. L., Sayers, G.: A technic for the preparation of isolated rat adrenal cells. Proc. Soc. exp. Biol. (N. Y.) 131, 1–4 (1969).
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol 4, 475–478 (1958).
Author information
Authors and Affiliations
Additional information
Supported by research grants from the U.S.P.H.S. (No. GM15872), and the Research Council of Rutgers University, and the National Science Foundation (No. GB7427 to Dr. George Sayers). We are indebted to Mrs. Jean A. Gibney, Miss Rose-Marie Ma, and Mrs. Mary Vegh for their excellent technical assistance.
Postdoctoral Trainee, U.S.P.H.S. Training Grant No. 5T01-GM00899-11.
Rights and permissions
About this article
Cite this article
Malamed, S., Sayers, G. & Swallow, R.L. Fine structure of trypsin-dissociated rat adrenal cells. Z. Zellforsch. 107, 447–453 (1970). https://doi.org/10.1007/BF00335433
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00335433