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Genetic studies of the ribosomal proteins in Escherichia coli

II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants

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Summary

Spectinomycin resistant (spc r) mutants were obtained by treating the cells of E. coli K12, W3637 with nitrosoguanidine. The compositions of ribosomal proteins were analyzed for six out of eleven such spc r-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 30s ribosomal subunit from all of the spc r-mutants was found to contain the altered 30-4 protein component, while no difference was detected in 50s ribosomal proteins between spc r and spc s bacteria.

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Abbreviations

CMC:

carboxymethyl cellulose

str :

streptomycin

spc :

spectinomycin

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan

    Satoshi Dekio & Renkichi Takata

Authors
  1. Satoshi Dekio
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  2. Renkichi Takata
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Communicated by H. G. Wittmann

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Dekio, S., Takata, R. Genetic studies of the ribosomal proteins in Escherichia coli . Molec. Gen. Genetics 105, 219–224 (1969). https://doi.org/10.1007/BF00337473

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  • DOI: https://doi.org/10.1007/BF00337473

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