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The complete amino-acid sequence of the rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis

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Summary

Summary

Several cDNA clones encoding the entire Rieske FeS-precursor protein of the chloroplast cytochrome b 6 f-complex have been isolated by high density plaque immunoscreening of a phage lambda gt11 cDNA expression library, made from poly A+-RNA of spinach seedlings. The identity of the cDNAs has been confirmed by N-terminal amino acid sequencing of the purified protein. The nucleotide sequence indicates a protein of 247 amino acid residues including a putative transit sequence of 68 amino acids corresponding to molecular masses of 26.3 kDa (precursor) and 18.8 kDa (mature protein; 179 amino acid residues). Alignteins of the sequence with sequences from Rieske FeS-proteins of respiratory electron transport chains, two of bacterial and three of mitochondrial origin, shows little sequence homology, but remarkable similarity in secondary structure including a putative N-terminal transmembrane segment of about 25 residues and the peptides CTHLGCV and CPCHGS in the C-terminal region of the protein that are involved in the binding of the Fe2S2-cluster.

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Authors and Affiliations

  1. Botanisches Institut der Ludwig-Maximilians-Universität, Menzinger Strasse 67, D-8000, München 19, Federal Republic of Germany

    J. Steppuhn, C. Rother, J. Hermans, T. Jansen & R. G. Herrmann

  2. Institut für Biochemie, Technische Universität Berlin, Franklinstrasse 29, D-1000, Berlin 10, Federal Republic of Germany

    J. Salnikow

  3. Institut für Botanik, Universität Regensburg, Universitätsstrasse 31, D-8400, Regensburg, Federal Republic of Germany

    G. Hauska

Authors
  1. J. Steppuhn
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  2. C. Rother
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  3. J. Hermans
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  4. T. Jansen
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  5. J. Salnikow
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  6. G. Hauska
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  7. R. G. Herrmann
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Communicated by C.P. Hollenberg

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Steppuhn, J., Rother, C., Hermans, J. et al. The complete amino-acid sequence of the rieske FeS-precursor protein from spinach chloroplasts deduced from cDNA analysis. Mol Gen Genet 210, 171–177 (1987). https://doi.org/10.1007/BF00337775

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  • DOI: https://doi.org/10.1007/BF00337775

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