Skip to main content
SpringerLink
Log in

A simple method for making new transducing lines of coliphage λ

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

A variety of novel transducing lines of phage λ can be obtained by induction of a mixed culture of abnormal lysogens. Such a culture is simply made by mass lysogenization of a host lacking the normal prophage attachment site.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andoh, T., Ozeki, H.: Suppressor gene su3+ of E. coli, a structural gene for tyrosine tRNA. Proc. nat. Acad. Sci. (Wash.) 59, 792–799 (1968)

    Google Scholar 

  • Bachman, B. J.: Pedigrees of some mutant strains of Escherichia coli K12. Bact. Rev. 36, 525–557 (1972)

    Google Scholar 

  • Campbell, A.: Chap. 2: Genetic structure. In: A. D. Hershey (ed.), The bacteriophage λ, p. 13–44. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Davis, R., Parkinson, J.: Deletion mutants of phage lambda. III. Physical structure of att ϕ. J. molec. Biol. 56, 403–423 (1971)

    Google Scholar 

  • Fiandt, M., Hradecna, Z., Lozeron, H., Szybalski, W.: Electron micrographic mapping of deletions, insertions, inversions, and homologies in the DNAs of coliphages lambda and phi 80. In: A. D. Hershey (ed.), The bacteriophage λ, p. 329–354. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Franklin, N.: The N operon of lambda: Extent and regulation as observed in fusions to the tryptophan operon of Escherichia coli. In: A. D. Hershey (ed.), The bacteriophage λ, p. 621–638. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Goldberg, A., Howe, M.: New mutants in the S cistron of bacteriophage lambda affecting host cell lysis. Virology 39, 200–202 (1969)

    Google Scholar 

  • Gottesman, M., Weisberg, R.: Chap. 6: Prophage insertion and excision. In: A. D. Hershey (ed.), The bacteriophage λ, p. 113–138. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Gottesman, S., Beckwith, J.: Directed transposition of the arabinose operon: a technique for the isolation of specialized transducing phages for any Escherichia coli gene. J. molec. Biol. 44, 117–127 (1969)

    Google Scholar 

  • Guarneros, G., Echols, H.: New mutants of bacteriophage λ with a specific defect in excision from the host chromosome. J. molec. Biol. 47, 565–574 (1970)

    Google Scholar 

  • Kayajanian, G.: Studies in the genetics of biotin-transducing, defective variants of bacteriophage λ. Virology 36, 30–41 (1968)

    Google Scholar 

  • Miller, J.: Experiments in molecular genetics. New York: Cold Spring Harbor Laboratory 1972

    Google Scholar 

  • Murray, N. E., Murray, K.: Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments. Nature (Lond.) 251, 476–481 (1974)

    Google Scholar 

  • Press, R., Glansdorff, N., Miner, P., DeVries, J., Kadner, R., Maas, W. K.: Isolation of transducing particles of ϕ80 bacteriophage that carry different regions of the Escherichia coli genome. Proc. nat. Acad. Sci. (Wash.) 68, 795–798 (1971)

    Google Scholar 

  • Reader, R., Siminovitch, L.: Lysis defective mutants of bacteriophage lambda: Genetics and physiology of S cistron mutants. Virology 43, 607–622 (1971)

    Google Scholar 

  • Shimada, K., Weisberg, R., Gottesman, M.: Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J. molec. Biol. 63, 483–503 (1972)

    Google Scholar 

  • Shimada, K., Weisberg, R., Gottesman, M.: Prophage lambda at unusual chromosomal locations. II. Mutations induced by bacteriophage lambda in Escherichia coli K12. J. molec. Biol. 80, 297–314 (1973)

    Google Scholar 

  • Sussman, R., Jacob, F.: Sur un système de répression thermosensible chez le bactériophage λ d'Escherichia coli. C. R. Acad. Sci. (Wash.) 254, 1517–1519 (1962)

    Google Scholar 

  • Szybalski, W., Herskowitz, I.: Lambda genetic elements. In: A. D. Hershey (ed.), The bacteriophage λ, p. 778–779. New York: Cold Spring Harbor Laboratory 1971

    Google Scholar 

  • Taylor, A., Trotter, C.: Linkage map of Escherichia coli strain K12. Bact. Rev. 36, 504–524 (1972)

    Google Scholar 

  • Thèze, J., Margarita, D.: Characterization of a defective λ bacteriophage transducing the threonine operon of Escherichia coli K12. Molec. gen. Genet. 132, 41–48 (1974)

    Google Scholar 

  • Weil, J., Cunningham, R., Martin III, R., Mitchell, E., Bolling, B.: Characteristics of λ p4, a λ derivative containing 9% excess DNA. Virology 50, 373–380 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, Maryland

    W. J. Schrenk & R. A. Weisberg

  2. Department of Biological Sciences, University of California at Santa Barbara, 93106, Santa Barbara, California, USA

    W. J. Schrenk

Authors
  1. W. J. Schrenk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. A. Weisberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by R. Devoret

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schrenk, W.J., Weisberg, R.A. A simple method for making new transducing lines of coliphage λ. Molec. gen. Genet. 137, 101–107 (1975). https://doi.org/10.1007/BF00341676

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00341676

Keywords

Search

Navigation