Summary
Transcription factor one (TF1), a protein synthesized after infection of B. subtilis by phage SPO1, is a specific inhibitor of SPO1 in vitro transcription. In this paper, we investigate the effect of TF1 on SPO1-specific in vitro protein synthesis, using SPO1 DNA or messenger RNA as templates. Protein synthesis is measured by incorporation of radioactive amino acids into acid insoluble form, synthesis of a phage-specific enzymatic activity, and analysis of radioactive polypeptides by acrylamide gel electrophoresis. TF1 is shown to inhibit the DNA-dependent synthesis of all SPO1 proteins which can be made in vitro, but to have no specific effect on RNA-dependent protein synthesis in vitro.
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Abbreviations
- TF1:
-
transcription factor one
- SDS:
-
sodium dodecyl sulfate
- CM:
-
chloramphenicol
References
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Communicated by W. Maas
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Shub, D.A., Johnson, G.G. Bacteriophage SPO1 DNA- and RNA-directed protein synthesis in vitro: The effect of TF1, a template-selective transcription inhibitor. Molec. gen. Genet. 137, 161–169 (1975). https://doi.org/10.1007/BF00341682
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DOI: https://doi.org/10.1007/BF00341682