Abstract
Using an improved Giemsa banding technique karyotypes were prepared from cells of two strains of laboratory rat (AS and Hooded Lister). Slides, aged for 7 days at room temperature were incubated in 2 x SSO at 60 °C for 3 hours and then exposed to 1% trypsin for 90 seconds at 10 ° C. Following Giemsa staining, consistent banding patterns were found in both early and late metaphase cells without loss of chromosome morphology. No major differences were found in the Giemsa banding patterns of the rat strains studied. Some variability in the banding pattern was observed for the small subterminal autosome (B5).
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References
Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science 130, 432–437 (1959).
Kurita, K., Sugiyama, T., Nishizuka, Y.: Cytogenetic studies on rat leukaemia induced by pulse doses of 7.12-Dimethylbenz(α)anthracene. Cancer Res. 28, 1738–1752 (1968).
McDougall, J. K.: Effects of adenoviruses on the chromosomes of normal human cells and cells trisomie for an E chromosome. Nature (Lond.) 225, 456–458 (1970).
Yosida, T. H., Sagai, T.: Banding pattern analysis of polymorphic karyotypes in the black rat by a new differential staining technique. Chromosoma (Berl.) 37, 387–394 (1972).
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Gallimore, P.H., Richardson, C.R. An improved banding technique exemplified in the karyotype analysis of two strains of rat. Chromosoma 41, 259–263 (1973). https://doi.org/10.1007/BF00344020
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DOI: https://doi.org/10.1007/BF00344020